Biomaterial Database

Use of galactose oxidase in labelling hydroxylysine glycosides of collagen. 1986

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Commercial preparations of galactose oxidase were shown to contain a proteolytic component active against collagen and collagen CNBr peptides which could not be inhibited by reagents such as EDTA, PMSF, PCMB and TLCK nor activated by Ca++ or Mn++. 30% of the total galactose oxidase activity could be recovered free of proteolytic activity in a simple one-step combined gel filtration/affinity chromatography procedure. The biological activity of such preparations was tested with reference to collagen hydroxylysine glycosides. It was shown that over 70% of the covalently linked tritium label introduced by KB3H4 reduction after galactose oxidase treatment was not associated with galactose but was present in the same non-specifically labelled components in control experiments. The enzyme could be used in a controlled manner to label hydroxylysine glycosides in collagen by immobilising it on agarose beads. In this form it was easy to store, re-usable and retained 95% of its original activity even after prolonged (18 h) incubation with substrates.

UI	MeSH Term	Description	Entries
D010446	Peptide Fragments	Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.	Peptide Fragment,Fragment, Peptide,Fragments, Peptide
D011865	Radioisotope Dilution Technique	Method for assessing flow through a system by injection of a known quantity of radionuclide into the system and monitoring its concentration over time at a specific point in the system. (From Dorland, 28th ed)	Radioisotope Dilution Technic,Dilution Technic, Radioisotope,Dilution Technics, Radioisotope,Dilution Technique, Radioisotope,Dilution Techniques, Radioisotope,Radioisotope Dilution Technics,Radioisotope Dilution Techniques,Technic, Radioisotope Dilution,Technics, Radioisotope Dilution,Technique, Radioisotope Dilution,Techniques, Radioisotope Dilution
D003094	Collagen	A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).	Avicon,Avitene,Collagen Felt,Collagen Fleece,Collagenfleece,Collastat,Dermodress,Microfibril Collagen Hemostat,Pangen,Zyderm,alpha-Collagen,Collagen Hemostat, Microfibril,alpha Collagen
D003488	Cyanogen Bromide	Cyanogen bromide (CNBr). A compound used in molecular biology to digest some proteins and as a coupling reagent for phosphoroamidate or pyrophosphate internucleotide bonds in DNA duplexes.	Bromide, Cyanogen
D004800	Enzymes, Immobilized	Enzymes which are immobilized on or in a variety of water-soluble or water-insoluble matrices with little or no loss of their catalytic activity. Since they can be reused continuously, immobilized enzymes have found wide application in the industrial, medical and research fields.	Immobilized Enzymes,Enzyme, Immobilized,Immobilized Enzyme
D005692	Galactose Oxidase	An enzyme that oxidizes galactose in the presence of molecular oxygen to D-galacto-hexodialdose. It is a copper protein. EC 1.1.3.9.	Oxidase, Galactose
D006901	Hydroxylysine	A hydroxylated derivative of the amino acid LYSINE that is present in certain collagens.	(2S,5R)-2,6-Diamino-5-hydroxyhexanoic Acid,2,6-Diamino-5-hydroxyhexanoic Acid,5-Hydroxylysine,Lysine, 5-Hydroxy-,2,6 Diamino 5 hydroxyhexanoic Acid,5 Hydroxylysine,Lysine, 5 Hydroxy
D014316	Tritium	The radioactive isotope of hydrogen also known as hydrogen-3. It contains two NEUTRONS and one PROTON in its nucleus and decays to produce low energy BETA PARTICLES.	Hydrogen-3,Hydrogen 3