BIOMAT Database Logo BIOMAT Database Logo

Differences in the fibril structure of corneal and tendon collagen. An electron microscopy and X-ray diffraction investigation. 1986

M Marchini, and M Morocutti, and A Ruggeri, and M H Koch, and A Bigi, and N Roveri

A detailed analysis of the D-period and axial electron density distribution of cornea and tendon collagen was carried out by means of X-ray diffraction and electron microscopy. Ultrastructural observations were made on replicas of freeze fractured and deep-etched specimens. Synchrotron radiation was used to obtain high resolution small angle X-ray diffraction patterns. The data provide evidence that D-period and intraperiod distances in cornea are shorter than in tendon collagen fibrils. The observed different banding observed is interpreted on the basis of the different morphological arrangement of the microfibrils in the two tissues: "helicoidal" in cornea and "straight" in tendon microfibrils.

UI MeSH Term Description Entries
D008854 Microscopy, Electron Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen. Electron Microscopy
D009928 Organ Specificity Characteristic restricted to a particular organ of the body, such as a cell type, metabolic response or expression of a particular protein or antigen. Tissue Specificity,Organ Specificities,Specificities, Organ,Specificities, Tissue,Specificity, Organ,Specificity, Tissue,Tissue Specificities
D011919 Rats, Inbred Strains Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding. August Rats,Inbred Rat Strains,Inbred Strain of Rat,Inbred Strain of Rats,Inbred Strains of Rats,Rat, Inbred Strain,August Rat,Inbred Rat Strain,Inbred Strain Rat,Inbred Strain Rats,Inbred Strains Rat,Inbred Strains Rats,Rat Inbred Strain,Rat Inbred Strains,Rat Strain, Inbred,Rat Strains, Inbred,Rat, August,Rat, Inbred Strains,Rats Inbred Strain,Rats Inbred Strains,Rats, August,Rats, Inbred Strain,Strain Rat, Inbred,Strain Rats, Inbred,Strain, Inbred Rat,Strains, Inbred Rat
D003094 Collagen A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH). Avicon,Avitene,Collagen Felt,Collagen Fleece,Collagenfleece,Collastat,Dermodress,Microfibril Collagen Hemostat,Pangen,Zyderm,alpha-Collagen,Collagen Hemostat, Microfibril,alpha Collagen
D003315 Cornea The transparent anterior portion of the fibrous coat of the eye consisting of five layers: stratified squamous CORNEAL EPITHELIUM; BOWMAN MEMBRANE; CORNEAL STROMA; DESCEMET MEMBRANE; and mesenchymal CORNEAL ENDOTHELIUM. It serves as the first refracting medium of the eye. It is structurally continuous with the SCLERA, avascular, receiving its nourishment by permeation through spaces between the lamellae, and is innervated by the ophthalmic division of the TRIGEMINAL NERVE via the ciliary nerves and those of the surrounding conjunctiva which together form plexuses. (Cline et al., Dictionary of Visual Science, 4th ed) Corneas
D005614 Freeze Fracturing Preparation for electron microscopy of minute replicas of exposed surfaces of the cell which have been ruptured in the frozen state. The specimen is frozen, then cleaved under high vacuum at the same temperature. The exposed surface is shadowed with carbon and platinum and coated with carbon to obtain a carbon replica. Fracturing, Freeze,Fracturings, Freeze,Freeze Fracturings
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013710 Tendons Fibrous bands or cords of CONNECTIVE TISSUE at the ends of SKELETAL MUSCLE FIBERS that serve to attach the MUSCLES to bones and other structures. Endotenon,Epotenon,Tendons, Para-Articular,Tendons, Paraarticular,Endotenons,Epotenons,Para-Articular Tendon,Para-Articular Tendons,Paraarticular Tendon,Paraarticular Tendons,Tendon,Tendon, Para-Articular,Tendon, Paraarticular,Tendons, Para Articular
D014961 X-Ray Diffraction The scattering of x-rays by matter, especially crystals, with accompanying variation in intensity due to interference effects. Analysis of the crystal structure of materials is performed by passing x-rays through them and registering the diffraction image of the rays (CRYSTALLOGRAPHY, X-RAY). (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Xray Diffraction,Diffraction, X-Ray,Diffraction, Xray,Diffractions, X-Ray,Diffractions, Xray,X Ray Diffraction,X-Ray Diffractions,Xray Diffractions
D046911 Macromolecular Substances Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure. Macromolecular Complexes,Macromolecular Compounds,Macromolecular Compounds and Complexes,Complexes, Macromolecular,Compounds, Macromolecular,Substances, Macromolecular

Related Publications

M Marchini, and M Morocutti, and A Ruggeri, and M H Koch, and A Bigi, and N Roveri
Spatial mapping of collagen fibril organisation in primate cornea-an X-ray diffraction investigation.
June 2004, Journal of structural biology,
M Marchini, and M Morocutti, and A Ruggeri, and M H Koch, and A Bigi, and N Roveri
X-ray diffraction investigations of collagen fibril structure in dermatosparactic lamb tissues.
December 1980, Laboratory investigation; a journal of technical methods and pathology,
M Marchini, and M Morocutti, and A Ruggeri, and M H Koch, and A Bigi, and N Roveri
An x-ray diffraction investigation of corneal structure in lumican-deficient mice.
July 2001, Investigative ophthalmology & visual science,
M Marchini, and M Morocutti, and A Ruggeri, and M H Koch, and A Bigi, and N Roveri
Light microscopy, electron microscopy, and X-ray diffraction analysis of glycerinated collagen fibers.
November 1983, Journal of ultrastructure research,
M Marchini, and M Morocutti, and A Ruggeri, and M H Koch, and A Bigi, and N Roveri
Crystal structure of an immunoglobulin molecule by x-ray diffraction and electron microscopy.
January 1972, Cold Spring Harbor symposia on quantitative biology,
M Marchini, and M Morocutti, and A Ruggeri, and M H Koch, and A Bigi, and N Roveri
The structure of amyloid fibrils by electron microscopy and X-ray diffraction.
January 1997, Advances in protein chemistry,
M Marchini, and M Morocutti, and A Ruggeri, and M H Koch, and A Bigi, and N Roveri
Preservation of corneal collagen fibril structure using low-temperature procedures for electron microscopy.
January 1986, Journal of ultrastructure and molecular structure research,
M Marchini, and M Morocutti, and A Ruggeri, and M H Koch, and A Bigi, and N Roveri
Macular corneal dystrophy: the macromolecular structure of the stroma observed using electron microscopy and synchrotron X-ray diffraction.
December 1989, Experimental eye research,
M Marchini, and M Morocutti, and A Ruggeri, and M H Koch, and A Bigi, and N Roveri
The investigation of the structure of collagen in growth process by X-ray analysis and electron microscopy.
December 1984, Biochimica et biophysica acta,
M Marchini, and M Morocutti, and A Ruggeri, and M H Koch, and A Bigi, and N Roveri
Electron microscopy and x-ray diffraction of bone.
February 1957, Biochimica et biophysica acta,
Copied contents to your clipboard!