OBJECTIVE To evaluate the ELISA kit for detection of IgG antibodies against Taenia solium cysticercus in humans, so as to provide a reference for its application in clinical practice. METHODS The sera collected from the patients with neurocysticercosis, echinococcosis, taeniasis and healthy people, respectively, were checked by ELISA for specific IgG antibodies against T. solium cysticercus, as described in the instruction of the kit. RESULTS Of the 30 patients with neurocysticercosis at active stage, 28 showed seropositive reaction, indicating a sensitivity of 93.33%. There were no positive reactions in the 100 healthy people. Two of the 42 persons with taeniasis were seropositive with an infection rate of 4.76%. Forty-one of the 60 persons with echinococcosis showed positive reactions, suggesting that the cross reaction rate of the kit with echinococcosis was 68.33%. CONCLUSIONS The assessed ELISA kit has a reasonably high sensitivity but a poor specificity, and the cross reaction rate with echinococcosis is very high. It is urgent to improve the sensitivity and specificity of the diagnostic kit for detection of T.solium cysticercosis in China.