BIOMAT Database Logo BIOMAT Database Logo

Activation of AMPK inhibits TGF-β1-induced airway smooth muscle cells proliferation and its potential mechanisms. 2018

Yilin Pan, and Lu Liu, and Shaojun Li, and Ke Wang, and Rui Ke, and Wenhua Shi, and Jian Wang, and Xin Yan, and Qianqian Zhang, and Qingting Wang, and Limin Chai, and Xinming Xie, and Manxiang Li
Department of Respiratory and Critical Care Medicine, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710061, P.R. China.

The aims of the present study were to examine signaling mechanisms underlying transforming growth factor β1 (TGF-β1)-induced airway smooth muscle cells (ASMCs) proliferation and to determine the effect of adenosine monophosphate-activated protein kinase (AMPK) activation on TGF-β1-induced ASMCs proliferation and its potential mechanisms. TGF-β1 reduced microRNA-206 (miR-206) level by activating Smad2/3, and this in turn up-regulated histone deacetylase 4 (HDAC4) and consequently increased cyclin D1 protein leading to ASMCs proliferation. Prior incubation of ASMCs with metformin induced AMPK activation and blocked TGF-β1-induced cell proliferation. Activation of AMPK slightly attenuated TGF-β1-induced miR-206 suppression, but dramatically suppressed TGF-β1-caused HDAC4 up-expression and significantly increased HDAC4 phosphorylation finally leading to reduction of up-regulated cyclin D1 protein expression. Our study suggests that activation of AMPK modulates miR-206/HDAC4/cyclin D1 signaling pathway, particularly targeting on HDAC4, to suppress ASMCs proliferation and therefore has a potential value in the prevention and treatment of asthma by alleviating airway remodeling.

UI MeSH Term Description Entries
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D006655 Histone Deacetylases Deacetylases that remove N-acetyl groups from amino side chains of the amino acids of HISTONES. The enzyme family can be divided into at least three structurally-defined subclasses. Class I and class II deacetylases utilize a zinc-dependent mechanism. The sirtuin histone deacetylases belong to class III and are NAD-dependent enzymes. Class I Histone Deacetylases,Class II Histone Deacetylases,HDAC Proteins,Histone Deacetylase,Histone Deacetylase Complexes,Complexes, Histone Deacetylase,Deacetylase Complexes, Histone,Deacetylase, Histone,Deacetylases, Histone
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D015151 Immunoblotting Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies. Dot Immunoblotting,Electroimmunoblotting,Immunoelectroblotting,Reverse Immunoblotting,Immunoblotting, Dot,Immunoblotting, Reverse,Dot Immunoblottings,Electroimmunoblottings,Immunoblottings,Immunoblottings, Dot,Immunoblottings, Reverse,Immunoelectroblottings,Reverse Immunoblottings
D015398 Signal Transduction The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway. Cell Signaling,Receptor-Mediated Signal Transduction,Signal Pathways,Receptor Mediated Signal Transduction,Signal Transduction Pathways,Signal Transduction Systems,Pathway, Signal,Pathway, Signal Transduction,Pathways, Signal,Pathways, Signal Transduction,Receptor-Mediated Signal Transductions,Signal Pathway,Signal Transduction Pathway,Signal Transduction System,Signal Transduction, Receptor-Mediated,Signal Transductions,Signal Transductions, Receptor-Mediated,System, Signal Transduction,Systems, Signal Transduction,Transduction, Signal,Transductions, Signal
D017207 Rats, Sprague-Dawley A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company. Holtzman Rat,Rats, Holtzman,Sprague-Dawley Rat,Rats, Sprague Dawley,Holtzman Rats,Rat, Holtzman,Rat, Sprague-Dawley,Sprague Dawley Rat,Sprague Dawley Rats,Sprague-Dawley Rats
D049109 Cell Proliferation All of the processes involved in increasing CELL NUMBER including CELL DIVISION. Cell Growth in Number,Cellular Proliferation,Cell Multiplication,Cell Number Growth,Growth, Cell Number,Multiplication, Cell,Number Growth, Cell,Proliferation, Cell,Proliferation, Cellular
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus
D053773 Transforming Growth Factor beta1 A subtype of transforming growth factor beta that is synthesized by a wide variety of cells. It is synthesized as a precursor molecule that is cleaved to form mature TGF-beta 1 and TGF-beta1 latency-associated peptide. The association of the cleavage products results in the formation a latent protein which must be activated to bind its receptor. Defects in the gene that encodes TGF-beta1 are the cause of CAMURATI-ENGELMANN SYNDROME. TGF-beta1,Transforming Growth Factor-beta1,TGF-beta-1,TGF-beta1 Latency-Associated Protein,TGF-beta1LAP,Transforming Growth Factor beta 1 Latency Associated Peptide,Transforming Growth Factor beta I,Latency-Associated Protein, TGF-beta1,TGF beta 1,TGF beta1 Latency Associated Protein,TGF beta1LAP
D055372 AMP-Activated Protein Kinases Intracellular signaling protein kinases that play a signaling role in the regulation of cellular energy metabolism. Their activity largely depends upon the concentration of cellular AMP which is increased under conditions of low energy or metabolic stress. AMP-activated protein kinases modify enzymes involved in LIPID METABOLISM, which in turn provide substrates needed to convert AMP into ATP. 5'-AMP-Activated Protein Kinase,AMP-Activated Kinase,AMP-Activated Protein Kinase,AMP-Activated Protein Kinase alpha Subunit,AMP-Activated Protein Kinase alpha Subunits,AMP-Activated Protein Kinase beta Subunit,AMP-Activated Protein Kinase beta Subunits,AMP-Activated Protein Kinase gamma Subunit,AMP-Activated Protein Kinase gamma Subunits,PRKAA,5' AMP Activated Protein Kinase,AMP Activated Kinase,AMP Activated Protein Kinase,AMP Activated Protein Kinase alpha Subunit,AMP Activated Protein Kinase alpha Subunits,AMP Activated Protein Kinase beta Subunit,AMP Activated Protein Kinase beta Subunits,AMP Activated Protein Kinase gamma Subunit,AMP Activated Protein Kinase gamma Subunits,AMP Activated Protein Kinases

Related Publications

Yilin Pan, and Lu Liu, and Shaojun Li, and Ke Wang, and Rui Ke, and Wenhua Shi, and Jian Wang, and Xin Yan, and Qianqian Zhang, and Qingting Wang, and Limin Chai, and Xinming Xie, and Manxiang Li
Apigenin inhibits TGF-β1-induced proliferation and migration of airway smooth muscle cells.
January 2015, International journal of clinical and experimental pathology,
Yilin Pan, and Lu Liu, and Shaojun Li, and Ke Wang, and Rui Ke, and Wenhua Shi, and Jian Wang, and Xin Yan, and Qianqian Zhang, and Qingting Wang, and Limin Chai, and Xinming Xie, and Manxiang Li
Protocatechuic acid inhibits TGF-β1-induced proliferation and migration of human airway smooth muscle cells.
January 2019, Journal of pharmacological sciences,
Yilin Pan, and Lu Liu, and Shaojun Li, and Ke Wang, and Rui Ke, and Wenhua Shi, and Jian Wang, and Xin Yan, and Qianqian Zhang, and Qingting Wang, and Limin Chai, and Xinming Xie, and Manxiang Li
SphK1/S1P mediates TGF-β1-induced proliferation of pulmonary artery smooth muscle cells and its potential mechanisms.
January 2019, Pulmonary circulation,
Yilin Pan, and Lu Liu, and Shaojun Li, and Ke Wang, and Rui Ke, and Wenhua Shi, and Jian Wang, and Xin Yan, and Qianqian Zhang, and Qingting Wang, and Limin Chai, and Xinming Xie, and Manxiang Li
Triptolide inhibits TGF-β1-induced cell proliferation in rat airway smooth muscle cells by suppressing Smad signaling.
February 2015, Experimental cell research,
Yilin Pan, and Lu Liu, and Shaojun Li, and Ke Wang, and Rui Ke, and Wenhua Shi, and Jian Wang, and Xin Yan, and Qianqian Zhang, and Qingting Wang, and Limin Chai, and Xinming Xie, and Manxiang Li
LXA4 inhibits TGF-β1-induced airway smooth muscle cells proliferation and migration by suppressing the Smad/YAP pathway.
May 2023, International immunopharmacology,
Yilin Pan, and Lu Liu, and Shaojun Li, and Ke Wang, and Rui Ke, and Wenhua Shi, and Jian Wang, and Xin Yan, and Qianqian Zhang, and Qingting Wang, and Limin Chai, and Xinming Xie, and Manxiang Li
Activation of AMPK inhibits pulmonary arterial smooth muscle cells proliferation.
June 2014, Experimental lung research,
Yilin Pan, and Lu Liu, and Shaojun Li, and Ke Wang, and Rui Ke, and Wenhua Shi, and Jian Wang, and Xin Yan, and Qianqian Zhang, and Qingting Wang, and Limin Chai, and Xinming Xie, and Manxiang Li
γ-Tocotrienol Inhibits TGF-β1-Induced Contractile Phenotype Expression of Human Airway Smooth Muscle Cells.
March 2017, Yonago acta medica,
Yilin Pan, and Lu Liu, and Shaojun Li, and Ke Wang, and Rui Ke, and Wenhua Shi, and Jian Wang, and Xin Yan, and Qianqian Zhang, and Qingting Wang, and Limin Chai, and Xinming Xie, and Manxiang Li
Platelet TGF-β1 inhibits the migration and proliferation of smooth muscle cells in aneurysms.
October 2022, Cytokine,
Yilin Pan, and Lu Liu, and Shaojun Li, and Ke Wang, and Rui Ke, and Wenhua Shi, and Jian Wang, and Xin Yan, and Qianqian Zhang, and Qingting Wang, and Limin Chai, and Xinming Xie, and Manxiang Li
Triptolide inhibits TGF-β1 induced proliferation and migration of rat airway smooth muscle cells by suppressing NF-κB but not ERK1/2.
September 2014, Immunology,
Yilin Pan, and Lu Liu, and Shaojun Li, and Ke Wang, and Rui Ke, and Wenhua Shi, and Jian Wang, and Xin Yan, and Qianqian Zhang, and Qingting Wang, and Limin Chai, and Xinming Xie, and Manxiang Li
Roxithromycin treatment inhibits TGF-β1-induced activation of ERK and AKT and down-regulation of caveolin-1 in rat airway smooth muscle cells.
August 2014, Respiratory research,
Copied contents to your clipboard!