-transparent.png){kind=logo}
The study reports morphological growth characteristics and chromosome analysis of neoplastic rat pleural mesothelial cells (RPMC) isolated from a mesothelioma-bearing rat. The pleural mesothelioma was induced by intrapleural injection of chrysotile fibers. Neoplastic RPMC were cultured by the standard methods used for normal RPMC. Neoplastic RPMC cultures had a population doubling time of 19 hr versus 30 hr for the normal cells. Plating efficiency in liquid medium was almost 100%. Cultures of neoplastic RPMC were anchorage-independent since 70% of the seeded cells formed colonies after one week; after the second week, colony size was enhanced but colony recovery was not. The serum dependence of neoplastic cells was less than that of the normal cells. 79 out of 100 metaphase cells analyzed had 41 to 43 chromosomes, and the modal number was 42 (38%). A large metacentric chromosome was observed in 77 of the 100 neoplastic metaphase cells analyzed, but not in any normal metaphase cells. In nude mice, the neoplastic RPMC were tumorigenic.
M C Jaurand, and
A Renier, and
A Van der Meeren, and
L Magne, and
M C Pinchon, and
J Bignon
January 1966,
Experimental cell research,
M C Jaurand, and
A Renier, and
A Van der Meeren, and
L Magne, and
M C Pinchon, and
J Bignon
May 2006,
Clinical cancer research : an official journal of the American Association for Cancer Research,
M C Jaurand, and
A Renier, and
A Van der Meeren, and
L Magne, and
M C Pinchon, and
J Bignon
June 1987,
Journal of neurology, neurosurgery, and psychiatry,
M C Jaurand, and
A Renier, and
A Van der Meeren, and
L Magne, and
M C Pinchon, and
J Bignon
January 1985,
Advances in experimental medicine and biology,
M C Jaurand, and
A Renier, and
A Van der Meeren, and
L Magne, and
M C Pinchon, and
J Bignon
November 1983,
Veterinarni medicina,
M C Jaurand, and
A Renier, and
A Van der Meeren, and
L Magne, and
M C Pinchon, and
J Bignon
April 1987,
In vitro cellular & developmental biology : journal of the Tissue Culture Association,
M C Jaurand, and
A Renier, and
A Van der Meeren, and
L Magne, and
M C Pinchon, and
J Bignon
June 2005,
Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology,
M C Jaurand, and
A Renier, and
A Van der Meeren, and
L Magne, and
M C Pinchon, and
J Bignon
February 1988,
The Journal of investigative dermatology,
M C Jaurand, and
A Renier, and
A Van der Meeren, and
L Magne, and
M C Pinchon, and
J Bignon
October 2010,
Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine],
M C Jaurand, and
A Renier, and
A Van der Meeren, and
L Magne, and
M C Pinchon, and
J Bignon
April 2006,
Shanghai kou qiang yi xue = Shanghai journal of stomatology,
