Biomaterial Database

Genetically encoded fluorescent voltage indicators: are we there yet? 2018

Jelena Platisa, and Vincent A Pieribone

The John B. Pierce Laboratory, Inc., New Haven, CT 06519, United States; Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06510, United States. Electronic address: jplatisa@jbpierce.org.

In order to understand how brain activity produces adaptive behavior we need large-scale, high-resolution recordings of neuronal activity. Fluorescent genetically encoded voltage indicators (GEVIs) offer the potential for these recordings to be performed chronically from targeted cells in a minimally invasive manner. As the number of GEVIs successfully tested for in vivo use grows, so has the number of open questions regarding the improvements that would facilitate broad adoption of this technology that surpasses mere 'proof of principle' studies. Our aim in this review is not to provide a status check of the current state of the field, as excellent publications covering this topic already exist. Here, we discuss specific questions regarding GEVI development and application that we think are crucial in achieving this goal.

UI	MeSH Term	Description	Entries
D008164	Luminescent Proteins	Proteins which are involved in the phenomenon of light emission in living systems. Included are the "enzymatic" and "non-enzymatic" types of system with or without the presence of oxygen or co-factors.	Bioluminescent Protein,Bioluminescent Proteins,Luminescent Protein,Photoprotein,Photoproteins,Protein, Bioluminescent,Protein, Luminescent,Proteins, Bioluminescent,Proteins, Luminescent
D001921	Brain	The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.	Encephalon
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D056969	Voltage-Sensitive Dye Imaging	Optical imaging techniques used for recording patterns of electrical activity in tissues by monitoring transmembrane potentials via FLUORESCENCE imaging with voltage-sensitive fluorescent dyes.	Action Potential Optical Mapping,Anatomic Electrical Activity Optical Mapping,Optical Action Potential Mapping,Optical Mapping, Action Potential,Optical Mapping, Anatomic Electrical Activity,Optical Mapping, Transmembrane Potential,Optical Transmembrane Potential Mapping,Optical Vm Mapping,Transmembrane Potential Optical Mapping,Imaging, Voltage-Sensitive Dye,Mapping, Optical Vm,Mappings, Optical Vm,Optical Vm Mappings,Vm Mapping, Optical,Vm Mappings, Optical,Voltage Sensitive Dye Imaging
D031541	Fluorescence Resonance Energy Transfer	A type of FLUORESCENCE SPECTROSCOPY using two FLUORESCENT DYES with overlapping emission and absorption spectra, which is used to indicate proximity of labeled molecules. This technique is useful for studying interactions of molecules and PROTEIN FOLDING.	Forster Resonance Energy Transfer