Biomaterial Database

[Lymphocyte migration induced by orciprenaline using the Boyden technic]. 1986

I Paegelow, and H Werner

The influence of the beta-sympathomimetic drug orciprenaline on the lymphocyte migration was investigated using the Boyden-technique. Orciprenaline was able to stimulate the migration of lymphocytes derived from lymph nodes of rats in a concentration range of 10(-10) to 10(-6) M. After 4.5 hours the maximum of the stimulation was reached. The supernatants collected after the stimulation of the lymph node cells with orciprenaline were also able to enhance the migration of PMN derived from guinea pigs of other lymphocyte cultures. The orciprenaline-induced stimulation of the lymphocyte migration was inhibited by propranolol (10(-9) M), whereas the addition of propranolol to the orciprenaline-evoked supernatants did not antagonize its stimulatory activity on the lymphocyte migration. After the concentration and the fractionation (Ultrogel AcA54) of the supernatants the migration stimulating activity could be demonstrated at the molecular weights of 40, 24, 18 and smaller than 12 kDa. It is supposed that sympathomimetic drugs are capable of inducing the lymph node cells to secrete mediators, which are responsible for the stimulation of the lymphocyte migration.

UI	MeSH Term	Description	Entries
D008198	Lymph Nodes	They are oval or bean shaped bodies (1 - 30 mm in diameter) located along the lymphatic system.	Lymph Node,Node, Lymph,Nodes, Lymph
D008214	Lymphocytes	White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.	Lymphoid Cells,Cell, Lymphoid,Cells, Lymphoid,Lymphocyte,Lymphoid Cell
D008722	Methods	A series of steps taken in order to conduct research.	Techniques,Methodological Studies,Methodological Study,Procedures,Studies, Methodological,Study, Methodological,Method,Procedure,Technique
D009921	Metaproterenol	A beta-2 adrenergic agonist used in the treatment of ASTHMA and BRONCHIAL SPASM.	Orciprenaline,Alotec,Alupent,Astmopent,Metaprel,Metaproterenol Polistirex,Metaproterenol Sulfate,Orciprenaline Sulfate,Polistirex, Metaproterenol
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D002634	Chemotaxis, Leukocyte	The movement of leukocytes in response to a chemical concentration gradient or to products formed in an immunologic reaction.	Leukotaxis,Leukocyte Chemotaxis
D006168	Guinea Pigs	A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.	Cavia,Cavia porcellus,Guinea Pig,Pig, Guinea,Pigs, Guinea
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D051381	Rats	The common name for the genus Rattus.	Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus