An apple NAC transcription factor enhances salt stress tolerance by modulating the ethylene response. 2018

Jian-Ping An, and Ji-Fang Yao, and Rui-Rui Xu, and Chun-Xiang You, and Xiao-Fei Wang, and Yu-Jin Hao
State Key Laboratory of Crop Biology, MOA Key Laboratory of Horticultural Crop Biology and Germplasm Innovation, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, Shandong, 271018, China.

It is known that ethylene signaling is involved in the regulation of the salt stress response. However, the molecular mechanism of ethylene-regulated salt stress tolerance remains largely unclear. In this study, an apple NAM ATAF CUC transcription factor, MdNAC047, was isolated and functionally characterized to be involved in ethylene-modulated salt tolerance. MdNAC047 gene was significantly induced by salt treatment and its overexpression conferred increased tolerance to salt stress and facilitated the release of ethylene. Quantitative real-time-PCR analysis demonstrated that overexpression of MdNAC047 increased the expression of ethylene-responsive genes. Electrophoretic mobility shift assay, yeast one-hybrid and dual-luciferase assays suggested that MdNAC047 directly binds to the MdERF3 (ETHYLENE RESPONSE FACTOR) promoter and activates its transcription. In addition, genetic analysis assays indicated that MdNAC047 regulates ethylene production at least partially in an MdERF3-dependent pathway. Overall, we found a novel 'MdNAC047-MdERF3-ethylene-salt tolerance' regulatory pathway, which provide new insight into the link between ethylene and salt stress.

UI MeSH Term Description Entries
D010940 Plant Proteins Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which PLANT PROTEINS, DIETARY is available. Plant Protein,Protein, Plant,Proteins, Plant
D005030 Ethylenes Derivatives of ethylene, a simple organic gas of biological origin with many industrial and biological use.
D013312 Stress, Physiological The unfavorable effect of environmental factors (stressors) on the physiological functions of an organism. Prolonged unresolved physiological stress can affect HOMEOSTASIS of the organism, and may lead to damaging or pathological conditions. Biotic Stress,Metabolic Stress,Physiological Stress,Abiotic Stress,Abiotic Stress Reaction,Abiotic Stress Response,Biological Stress,Metabolic Stress Response,Physiological Stress Reaction,Physiological Stress Reactivity,Physiological Stress Response,Abiotic Stress Reactions,Abiotic Stress Responses,Abiotic Stresses,Biological Stresses,Biotic Stresses,Metabolic Stress Responses,Metabolic Stresses,Physiological Stress Reactions,Physiological Stress Responses,Physiological Stresses,Reaction, Abiotic Stress,Reactions, Abiotic Stress,Response, Abiotic Stress,Response, Metabolic Stress,Stress Reaction, Physiological,Stress Response, Metabolic,Stress Response, Physiological,Stress, Abiotic,Stress, Biological,Stress, Biotic,Stress, Metabolic
D014157 Transcription Factors Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process. Transcription Factor,Factor, Transcription,Factors, Transcription
D055049 Salt Tolerance The ability of organisms to sense and adapt to high concentrations of salt in their growth environment. Salt-Tolerance,Saline-Tolerance,Salinity Tolerance,Salt Adaptation,Salt Adaption,Salt-Adaption,Adaptation, Salt,Adaption, Salt,Saline Tolerance,Salinity Tolerances,Salt Adaptations,Salt Adaptions,Salt Tolerances,Tolerance, Salinity,Tolerance, Salt
D018506 Gene Expression Regulation, Plant Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in plants. Plant Gene Expression Regulation,Regulation of Gene Expression, Plant,Regulation, Gene Expression, Plant
D024202 Electrophoretic Mobility Shift Assay An electrophoretic technique for assaying the binding of one compound to another. Typically one compound is labeled to follow its mobility during electrophoresis. If the labeled compound is bound by the other compound, then the mobility of the labeled compound through the electrophoretic medium will be retarded. Gelshift Analysis,Mobility Shift Assay,Band Shift Mobility Assay,Bandshift Mobility Assay,EMSA Electrophoretic Technique,Gel Retardation Assay,Gel Shift Analysis,Supershift Mobility Assay,Analyses, Gel Shift,Analysis, Gel Shift,Assay, Bandshift Mobility,Assay, Gel Retardation,Assay, Mobility Shift,Assay, Supershift Mobility,Assays, Bandshift Mobility,Assays, Gel Retardation,Assays, Mobility Shift,Assays, Supershift Mobility,Bandshift Mobility Assays,EMSA Electrophoretic Techniques,Electrophoretic Technique, EMSA,Electrophoretic Techniques, EMSA,Gel Retardation Assays,Gel Shift Analyses,Mobility Assay, Bandshift,Mobility Assay, Supershift,Mobility Assays, Bandshift,Mobility Assays, Supershift,Mobility Shift Assays,Supershift Mobility Assays,Technique, EMSA Electrophoretic,Techniques, EMSA Electrophoretic
D027845 Malus A plant genus in the family ROSACEAE, order Rosales, subclass Rosidae. It is best known as a source of the edible fruit (apple) and is cultivated in temperate climates worldwide. Apple,Apple Tree,Crab Apple,Malus domestica,Apple Trees,Apple, Crab,Apples,Apples, Crab,Crab Apples,Tree, Apple,Trees, Apple

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