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Free and Apo B-associated Lpa-specific protein in human serum. 1987

A Gries, and J Nimpf, and M Nimpf, and H Wurm, and G M Kostner

The distribution of Lpa-specific protein (Apo A) among various fractions of human serum was investigated. The following results were obtained. In very low density lipoproteins (VLDL) of fasting human serum Apo A cannot be detected. Most of Apo A is complexed to LDL forming lipoprotein a (Lpa) present in the density fraction of 1.006-1.125 g/ml. Only approximately 5% of Apo A are found in the d greater than 1.125 bottom fraction after ultracentrifugation. Phosphotungstate-Mg (PTA), heparin-Mn (Hep) and dextran sulfate-Mg (DS) precipitate approximately 95% of serum Lpa together with the Apo B containing lipoproteins. Also monospecific antibodies against Apo B do not precipitate all Lpa and leave approximately 3% of Apo A in the supernatant. With polyethylene glycol (PEG) on the other hand, virtually all Lpa is removed since this reagent also precipitates Lpa and Apo A. Apo A of the bottom fraction after ultracentrifugation and of the supernatant after selective precipitation is not stainable with Sudan black and probably represents a protein which is not associated with lipids.

UI MeSH Term Description Entries
D007120 Immunochemistry Field of chemistry that pertains to immunological phenomena and the study of chemical reactions related to antigen stimulation of tissues. It includes physicochemical interactions between antigens and antibodies.
D008074 Lipoproteins Lipid-protein complexes involved in the transportation and metabolism of lipids in the body. They are spherical particles consisting of a hydrophobic core of TRIGLYCERIDES and CHOLESTEROL ESTERS surrounded by a layer of hydrophilic free CHOLESTEROL; PHOSPHOLIPIDS; and APOLIPOPROTEINS. Lipoproteins are classified by their varying buoyant density and sizes. Circulating Lipoproteins,Lipoprotein,Lipoproteins, Circulating
D008297 Male Males
D011232 Chemical Precipitation The formation of a solid in a solution as a result of a chemical reaction or the aggregation of soluble substances into complexes large enough to fall out of solution. Precipitation, Chemical
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000293 Adolescent A person 13 to 18 years of age. Adolescence,Youth,Adolescents,Adolescents, Female,Adolescents, Male,Teenagers,Teens,Adolescent, Female,Adolescent, Male,Female Adolescent,Female Adolescents,Male Adolescent,Male Adolescents,Teen,Teenager,Youths
D000328 Adult A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available. Adults
D001054 Apolipoproteins A Structural proteins of the alpha-lipoproteins (HIGH DENSITY LIPOPROTEINS), including APOLIPOPROTEIN A-I and APOLIPOPROTEIN A-II. They can modulate the activity of LECITHIN CHOLESTEROL ACYLTRANSFERASE. These apolipoproteins are low in atherosclerotic patients. They are either absent or present in extremely low plasma concentration in TANGIER DISEASE. Apo-A,ApoA
D014461 Ultracentrifugation Centrifugation with a centrifuge that develops centrifugal fields of more than 100,000 times gravity. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

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