Aberrant crypt foci are regionally affected by zinc treatment in a 1,2-dimethylhydrazine induced colon carcinogenesis model. 2018

Hichem Moulahoum, and Belkacem Mohamed Amine Boumaza, and Meriem Ferrat, and Andras-Laszlo Nagy, and Diana Elena Olteanu, and Abdelkader Bounaama, and Simona Clichici
Laboratory of Cell and Molecular Biology, Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene (USTHB), Algiers, Algeria; Biochemistry Department, Faculty of Sciences, Ege University, Izmir, Turkey. Electronic address: hmoulahoum@usthb.dz.

Zinc is a trace element widely known for its marked antioxidant properties. To gain more insight into the site- and time- specific mechanisms by which it induces chemoprevention, this study was elaborated over a pre-cancerous model of colon carcinogenesis. Colon cancer was induced by 1,2-dimethylhydrazine (DMH) in mice (20 mg/kg for 2 weeks) and groups of animals were supplemented with or without zinc sulfate (ZnSO4, 200 mg/L) in drinking water for 4, 10 or 14 weeks. Colon tissues were collected for pathological observation, analyzing aberrant crypt (AC) and aberrant crypt foci (ACF) formations, multiplicity and distribution. Similarly, histological assessment and mucin production, as well as oxidative stress markers estimation was performed for the different groups. Results showed a significant increase in ACF and AC numbers, ACF multiplicity and demonstrated stronger distal occurrence than in the proximal after DHM administration. Histopathological analysis presented marked structural alterations and mucin loss in the distal than the proximal colons. A significant increase in myeloperoxidase (MPO), nitric oxide (NO), L-ornithine and malondialdehyde (MDA) levels was observed followed by a significant decrease in antioxidant markers (superoxide dismutase (SOD), catalase (CAT) and reduced glutathione (GSH)). Oral ZnSO4 supplementation (continuous or partial) induced significant decrease in ACF, AC numbers and multiplicity, restored histological architecture and mucin production, and a significant decrease in proinflammatory markers while it reduced antioxidants to normal levels. From this study, insight was obtained on the use of ZnSO4 as a chemopreventive agent and shed light on its potential, as a supplement in nutraceutical approaches.

UI MeSH Term Description Entries
D009374 Neoplasms, Experimental Experimentally induced new abnormal growth of TISSUES in animals to provide models for studying human neoplasms. Experimental Neoplasms,Experimental Neoplasm,Neoplasm, Experimental
D011230 Precancerous Conditions Pathological conditions that tend eventually to become malignant. Preneoplastic Conditions,Condition, Preneoplastic,Conditions, Preneoplastic,Preneoplastic Condition,Condition, Precancerous,Conditions, Precancerous,Precancerous Condition
D003110 Colonic Neoplasms Tumors or cancer of the COLON. Cancer of Colon,Colon Adenocarcinoma,Colon Cancer,Cancer of the Colon,Colon Neoplasms,Colonic Cancer,Neoplasms, Colonic,Adenocarcinoma, Colon,Adenocarcinomas, Colon,Cancer, Colon,Cancer, Colonic,Cancers, Colon,Cancers, Colonic,Colon Adenocarcinomas,Colon Cancers,Colon Neoplasm,Colonic Cancers,Colonic Neoplasm,Neoplasm, Colon,Neoplasm, Colonic,Neoplasms, Colon
D004798 Enzymes Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however CATALYTIC RNA and CATALYTIC DNA molecules have also been identified. Biocatalyst,Enzyme,Biocatalysts
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000975 Antioxidants Naturally occurring or synthetic substances that inhibit or retard oxidation reactions. They counteract the damaging effects of oxidation in animal tissues. Anti-Oxidant,Antioxidant,Antioxidant Activity,Endogenous Antioxidant,Endogenous Antioxidants,Anti-Oxidant Effect,Anti-Oxidant Effects,Anti-Oxidants,Antioxidant Effect,Antioxidant Effects,Activity, Antioxidant,Anti Oxidant,Anti Oxidant Effect,Anti Oxidant Effects,Anti Oxidants,Antioxidant, Endogenous,Antioxidants, Endogenous
D016588 Anticarcinogenic Agents Agents that reduce the frequency or rate of spontaneous or induced tumors independently of the mechanism involved. Anti-Carcinogenic Agents,Anti-Carcinogenic Drugs,Anti-Carcinogenic Effect,Anti-Carcinogenic Effects,Anticarcinogenic Drugs,Anticarcinogenic Effect,Anticarcinogenic Effects,Anticarcinogens,Agents, Anti-Carcinogenic,Agents, Anticarcinogenic,Anti Carcinogenic Agents,Anti Carcinogenic Drugs,Anti Carcinogenic Effect,Anti Carcinogenic Effects,Drugs, Anti-Carcinogenic,Drugs, Anticarcinogenic,Effect, Anti-Carcinogenic,Effect, Anticarcinogenic,Effects, Anti-Carcinogenic,Effects, Anticarcinogenic
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D058739 Aberrant Crypt Foci Clusters of colonic crypts that appear different from the surrounding mucosa when visualized after staining. They are of interest as putative precursors to colorectal adenomas and potential biomarkers for colorectal carcinoma. Crypt Foci, Aberrant,Foci, Aberrant Crypt
D018384 Oxidative Stress A disturbance in the prooxidant-antioxidant balance in favor of the former, leading to potential damage. Indicators of oxidative stress include damaged DNA bases, protein oxidation products, and lipid peroxidation products (Sies, Oxidative Stress, 1991, pxv-xvi). Anti-oxidative Stress,Antioxidative Stress,DNA Oxidative Damage,Nitro-Oxidative Stress,Oxidative Cleavage,Oxidative DNA Damage,Oxidative Damage,Oxidative Injury,Oxidative Nitrative Stress,Oxidative Stress Injury,Oxidative and Nitrosative Stress,Stress, Oxidative,Anti oxidative Stress,Anti-oxidative Stresses,Antioxidative Stresses,Cleavage, Oxidative,DNA Damage, Oxidative,DNA Oxidative Damages,Damage, DNA Oxidative,Damage, Oxidative,Damage, Oxidative DNA,Injury, Oxidative,Injury, Oxidative Stress,Nitrative Stress, Oxidative,Nitro Oxidative Stress,Nitro-Oxidative Stresses,Oxidative Cleavages,Oxidative DNA Damages,Oxidative Damage, DNA,Oxidative Damages,Oxidative Injuries,Oxidative Nitrative Stresses,Oxidative Stress Injuries,Oxidative Stresses,Stress Injury, Oxidative,Stress, Anti-oxidative,Stress, Antioxidative,Stress, Nitro-Oxidative,Stress, Oxidative Nitrative,Stresses, Nitro-Oxidative

Related Publications

Hichem Moulahoum, and Belkacem Mohamed Amine Boumaza, and Meriem Ferrat, and Andras-Laszlo Nagy, and Diana Elena Olteanu, and Abdelkader Bounaama, and Simona Clichici
May 2006, Carcinogenesis,
Hichem Moulahoum, and Belkacem Mohamed Amine Boumaza, and Meriem Ferrat, and Andras-Laszlo Nagy, and Diana Elena Olteanu, and Abdelkader Bounaama, and Simona Clichici
January 2000, Nutrition and cancer,
Hichem Moulahoum, and Belkacem Mohamed Amine Boumaza, and Meriem Ferrat, and Andras-Laszlo Nagy, and Diana Elena Olteanu, and Abdelkader Bounaama, and Simona Clichici
January 1997, Cancer detection and prevention,
Hichem Moulahoum, and Belkacem Mohamed Amine Boumaza, and Meriem Ferrat, and Andras-Laszlo Nagy, and Diana Elena Olteanu, and Abdelkader Bounaama, and Simona Clichici
June 1993, Cancer research,
Hichem Moulahoum, and Belkacem Mohamed Amine Boumaza, and Meriem Ferrat, and Andras-Laszlo Nagy, and Diana Elena Olteanu, and Abdelkader Bounaama, and Simona Clichici
June 1991, Carcinogenesis,
Hichem Moulahoum, and Belkacem Mohamed Amine Boumaza, and Meriem Ferrat, and Andras-Laszlo Nagy, and Diana Elena Olteanu, and Abdelkader Bounaama, and Simona Clichici
January 1994, Cancer letters,
Hichem Moulahoum, and Belkacem Mohamed Amine Boumaza, and Meriem Ferrat, and Andras-Laszlo Nagy, and Diana Elena Olteanu, and Abdelkader Bounaama, and Simona Clichici
January 2005, Cell biology and toxicology,
Hichem Moulahoum, and Belkacem Mohamed Amine Boumaza, and Meriem Ferrat, and Andras-Laszlo Nagy, and Diana Elena Olteanu, and Abdelkader Bounaama, and Simona Clichici
November 2008, Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas,
Hichem Moulahoum, and Belkacem Mohamed Amine Boumaza, and Meriem Ferrat, and Andras-Laszlo Nagy, and Diana Elena Olteanu, and Abdelkader Bounaama, and Simona Clichici
January 2009, Current opinion in gastroenterology,
Hichem Moulahoum, and Belkacem Mohamed Amine Boumaza, and Meriem Ferrat, and Andras-Laszlo Nagy, and Diana Elena Olteanu, and Abdelkader Bounaama, and Simona Clichici
January 2010, Oncology research,
Copied contents to your clipboard!