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Immunomodulation induced through ornithine decarboxylase DNA immunization in Balb/c mice infected with Leishmania donovani. 2018
Akhilesh Kumar, and
Manas Ranjan Dikhit, and
Ajay Amit, and
Amir Zaidi, and
Raj Kishor Pandey, and
Ashish Kumar Singh, and
Shashi S Suman, and
Vahab Ali, and
Vidya Nand Rabi Das, and
Krishna Pandey, and
Vikas Kumar, and
Shubhankar Kumar Singh, and
Shyam Narayan, and
Hirendra Kumar Chourasia, and
Pradeep Das, and
Sanjiva Bimal
Division of Immunology, Rajendra Memorial Research Institute of Medical Sciences, Patna 800007, India.
We report here a Leishmania donovani ornithine decarboxylase (Ld-ODC) gene used as a DNA vaccine against visceral leishmaniasis in a murine Balb/c mouse model. This study also evaluated the possible mechanism of action directed by this candidate. We found a Th1 immune response after immunization using an Ld-ODC DNA vaccine, with results based on the rearrangement of TCR-V-α-2, proliferation of Carboxy fluorescein Succinimidyle ester positive T cells, which were able to produce cytokines such as TNF-α, IFN-γ, IL-12 and IL-2, but not IL-4, IL-5, IL-6 and IL-10, and modulations of the STAT-1 and p38 MAP kinase signaling pathways. The results were corroborated with the reduction in the amastigote proliferation and parasite killing in spleens after infection in vitro. We conclude this study suggesting that the Ld-ODC DNA construct could be a new vaccine candidate against visceral leishmaniasis.
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