The malaria parasite Plasmodium falciparum Sortilin is essential for merozoite formation and apical complex biogenesis. 2018

Stéphanie Hallée, and Natalie A Counihan, and Kathryn Matthews, and Tania F de Koning-Ward, and Dave Richard
Centre de recherche en infectiologie, CHU de Québec-Université Laval, Quebec City, QC, Canada.

The inner membrane complex and the apical secretory organelles are defining features of apicomplexan parasites. Despite their critical roles, the mechanisms behind the biogenesis of these structures in the malaria parasite Plasmodium falciparum are still poorly defined. We here show that decreasing expression of the P. falciparum homologue of the conserved endolysomal escorter Sortilin-VPS10 prevents the formation of the inner membrane complex and abrogates the generation of new merozoites. Moreover, protein trafficking to the rhoptries, the micronemes, and the dense granules is disrupted, which leads to the accumulation of apical complex proteins in the endoplasmic reticulum and the parasitophorous vacuole. We further show that protein export to the erythrocyte and transport through the constitutive secretory pathway are functional. Taken together, our results suggest that the malaria parasite P. falciparum Sortilin has potentially broader functions than most of its other eukaryotic counterparts.

UI MeSH Term Description Entries
D010963 Plasmodium falciparum A species of protozoa that is the causal agent of falciparum malaria (MALARIA, FALCIPARUM). It is most prevalent in the tropics and subtropics. Plasmodium falciparums,falciparums, Plasmodium
D001678 Organelle Biogenesis The natural growth and development within living CELLS. Mitochondrial Biogenesis,Biogeneses, Organelle,Biogenesis, Mitochondrial,Biogenesis, Organelle,Organelle Biogeneses
D052940 Merozoites Uninuclear cells or a stage in the life cycle of sporozoan protozoa. Merozoites, released from ruptured multinucleate SCHIZONTS, enter the blood stream and infect the ERYTHROCYTES. Merozoite
D055785 Gene Knockdown Techniques The artificial induction of GENE SILENCING by the use of RNA INTERFERENCE to reduce the expression of a specific gene. It includes the use of DOUBLE-STRANDED RNA, such as SMALL INTERFERING RNA and RNA containing HAIRPIN LOOP SEQUENCE, and ANTI-SENSE OLIGONUCLEOTIDES. Gene Knock Down Techniques,Gene Knock Down,Gene Knock-Down,Gene Knock-Down Techniques,Gene Knockdown,Gene Knock Downs,Gene Knock-Down Technique,Gene Knock-Downs,Gene Knockdown Technique,Gene Knockdowns,Knock Down, Gene,Knock Downs, Gene,Knock-Down Technique, Gene,Knock-Down Techniques, Gene,Knock-Down, Gene,Knock-Downs, Gene,Knockdown Technique, Gene,Knockdown Techniques, Gene,Knockdown, Gene,Knockdowns, Gene,Technique, Gene Knock-Down,Technique, Gene Knockdown,Techniques, Gene Knock-Down,Techniques, Gene Knockdown
D021381 Protein Transport The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport. Cellular Protein Targeting,Gated Protein Transport,Protein Targeting, Cellular,Protein Translocation,Transmembrane Protein Transport,Vesicular Protein Transport,Protein Localization Processes, Cellular,Protein Sorting,Protein Trafficking,Protein Transport, Gated,Protein Transport, Transmembrane,Protein Transport, Vesicular,Traffickings, Protein
D033942 Adaptor Proteins, Vesicular Transport A class of proteins involved in the transport of molecules via TRANSPORT VESICLES. They perform functions such as binding to the cell membrane, capturing cargo molecules and promoting the assembly of CLATHRIN. The majority of adaptor proteins exist as multi-subunit complexes, however monomeric varieties have also been found. Clathrin Adaptor,Clathrin Adaptor Protein Complex,Clathrin Assembly Protein,Clathrin Assembly Protein Complex,Clathrin Assembly Proteins,Clathrin-Associated Adaptor,Clathrin-Associated Protein,Vesicular Transport Adaptor Protein,Vesicular Transport Adaptor Protein Complex,Vesicular Transport Adaptor Proteins,Adaptor Protein Complexes, Vesicular Transport,Clathrin Adaptor Protein Complexes,Clathrin Adaptors,Clathrin Assembly Protein Complexes,Clathrin-Associated Adaptors,Clathrin-Associated Proteins,Vesicular Transport Adaptor Protein Complexes,Adaptor, Clathrin,Adaptor, Clathrin-Associated,Adaptors, Clathrin,Adaptors, Clathrin-Associated,Assembly Protein, Clathrin,Assembly Proteins, Clathrin,Clathrin Associated Adaptor,Clathrin Associated Adaptors,Clathrin Associated Protein,Clathrin Associated Proteins,Protein, Clathrin Assembly,Protein, Clathrin-Associated

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