The purpose of this study was to determine if changes in intra-muscular myoglobin concentration accompany histochemical and enzymatic adaptations to supra-maximal exercise training. Subjects were assigned to either a training group (N = 11), who trained 2 to 3 times weekly for 6 wk, or a control group (N = 6). Training progressed from two 15-s and two 30-s "all-out" sprints on a cycle ergometer during week 1 to six 15-s and six 30-s bouts per session during week 6. The Wingate test was performed before and after the 6 wk, but performance variables were not changed in either group. In the training group, peak lactate after the Wingate test was significantly higher after training. No significant changes in enzyme activities, myoglobin concentration, or fiber-type frequency were observed in the control group. In contrast, in the training group, the percent fast twitch oxidative fibers increased, myoglobin decreased, and both citrate synthase and phosphofructokinase activities increased (P less than 0.05). The results suggest that muscle myoglobin concentration is not increased by 6 wk of supra-maximal exercise training and that such training induces cellular adaptations without accompanying performance changes. Alternatively, the Wingate test is not a sensitive test of adaptations to the training.