Characterization of isolates of Bordetella bronchiseptica from horses. 2018

Rajesh Kumar Vaid, and Karuppusamy Shanmugasundaram, and Taruna Anand, and Bidhan Chandra Bera, and Mamta Tigga, and Ramesh Dedar, and Thachamvally Riyesh, and Shashank Bardwaj, and Nitin Virmani, and Bhupendra Nath Tripathi, and Rajkumar Singh
Bacteriology Laboratory, National Centre for Veterinary Type Cultures, National Research Centre on Equines, 125 001 Haryana, India.

Bordetella bronchiseptica is a well-known Gram-negative bacterial pathogen causing a plethora of diseases in different animals. Although its infection has been reported from pigs and dogs in India, no report of B. bronchiseptica from horses is described. We report for the first time, isolation, identification and characterization of strains of B. bronchiseptica from respiratory infection in horses from different states in India. The antimicrobial susceptibility testing showed resistance to penicillins, ceftazidime, and chloramphanicol. The virulence capability of the strains was confirmed by sequencing genes such as adenylate cyclase toxin (cyaA), bordetella virulence gene (bvgA) and by PCR detection of flagellin gene (fla). We demonstrate the involvement of B. bronchiseptica strains in respiratory tract infection in horses in India.

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