Biomaterial Database

A quantitative thin layer chromatography method for the analysis of aflatoxins, ochratoxin A, zearalenone, T-2 toxin and sterigmatocystin in foodstuffs. 1985

M O Tapia

Departamento de Producción Animal, INTA Balcarce, Buenos Aires, Argentina.

A simple multimycotoxin analytical method was devised for the assay of 8 mycotoxins in foodstuff samples. The procedure consisted in the extraction of the samples with acetonitrile-4% KCl with the addition of HCl, defating with hexane and final extraction with methylene chloride. The mycotoxins were separated by bidimensional thin layer chromatography. The recovery was higher than 80% for all the mycotoxins. The limits of detection were 1.2 micrograms aflatoxins, 5 micrograms ochratoxin A, 20 micrograms sterigmatocystin, 100 micrograms T-2 toxin and 100 micrograms zearalenone/kg.

UI	MeSH Term	Description	Entries
D009183	Mycotoxins	Toxic compounds produced by FUNGI.	Fungal Toxins,Mycotoxin,Toxins, Fungal
D009793	Ochratoxins	Isocoumarins found in ASPERGILLUS OCHRACEUS and other FUNGI. Ochratoxin contaminated FOOD has been responsible for cases of FOODBORNE DISEASES.	Ochratoxin
D002523	Edible Grain	SEEDS used as a major nutritional source, most often the grain from the POACEAE family.	Cereals,Cereal Grain,Cereal,Cereal Grains,Edible Grains,Grain, Cereal,Grain, Edible,Grains, Cereal,Grains, Edible
D002855	Chromatography, Thin Layer	Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Chromatography, Thin-Layer,Thin Layer Chromatography,Chromatographies, Thin Layer,Chromatographies, Thin-Layer,Thin Layer Chromatographies,Thin-Layer Chromatographies,Thin-Layer Chromatography
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000348	Aflatoxins	Furano-furano-benzopyrans that are produced by ASPERGILLUS from STERIGMATOCYSTIN. They are structurally related to COUMARINS and easily oxidized to an epoxide form to become ALKYLATING AGENTS. Members of the group include AFLATOXIN B1; aflatoxin B2, aflatoxin G1, aflatoxin G2; AFLATOXIN M1; and aflatoxin M2.	Aflatoxin
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000821	Animal Feed	Foodstuff used especially for domestic and laboratory animals, or livestock.	Fodder,Animal Feeds,Feed, Animal,Feeds, Animal,Fodders
D013605	T-2 Toxin	A potent mycotoxin produced in feedstuffs by several species of the genus FUSARIUM. It elicits a severe inflammatory reaction in animals and has teratogenic effects.	Fusaritoxin,Fusariotoxin,T-2 Toxin, alpha,4 beta-Isomer,T2 Toxin,T 2 Toxin,Toxin, T-2,Toxin, T2
D015025	Zearalenone	(S-(E))-3,4,5,6,8,10-Hexahydro-14,16-dihydroxy-3-methyl-1H-2-benzoxacyclotetradecin-1,7(8H)-dione. One of a group of compounds known under the general designation of resorcylic acid lactones. Cis, trans, dextro and levo forms have been isolated from the fungus Gibberella zeae (formerly Fusarium graminearum). They have estrogenic activity, cause toxicity in livestock as feed contaminant, and have been used as anabolic or estrogen substitutes.	F-2 Toxin,F2 Toxin,F 2 Toxin,Toxin, F-2,Toxin, F2