BIOMAT Database Logo BIOMAT Database Logo

Generation of haploid spermatids from chicken embryonal primordial germ cells. 2018

Meng Ji, and Shuang Tang, and Wenhua Pei, and Mingming Ning, and Yuehui Ma, and Xiangchen Li, and Weijun Guan
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China.

In vitro production of functional spermatids has special significance in the research of spermatogenesis and the treatment of male infertility. Primordial germ cells (PGCs) are the precursors of oocyte and sperm, which generate the totipotent cells. Studies have shown that PGCs have the potential ability to develop meiotic spermatids in vitro. Here we have shown that retinoic acid (RA) leads to PGC differentiation, and SCF can improve the efficiency of induction. We indicate an efficient approach to produce haploid spermatids from chicken PGCs in the presence of RA and stem cell factor (SCF). Real-time RT-PCR assays showed that RA and SCF induced a remarkable increase in expression of SYCP1, ACR, BOULE and DCM1 of meiotic germ cells and haploid germ cells, respectively. DNA content assays revealed that RA and SCF induced a remarkable increase of haploid cells. This study provides a theoretical basis and a great animal model for spermatogenesis study.

UI MeSH Term Description Entries
D008297 Male Males
D008540 Meiosis A type of CELL NUCLEUS division, occurring during maturation of the GERM CELLS. Two successive cell nucleus divisions following a single chromosome duplication (S PHASE) result in daughter cells with half the number of CHROMOSOMES as the parent cells. M Phase, Meiotic,Meiotic M Phase,M Phases, Meiotic,Meioses,Meiotic M Phases,Phase, Meiotic M,Phases, Meiotic M
D002642 Chick Embryo The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching. Embryo, Chick,Chick Embryos,Embryos, Chick
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D005260 Female Females
D006238 Haploidy The chromosomal constitution of cells, in which each type of CHROMOSOME is represented once. Symbol: N. Haploid,Haploid Cell,Cell, Haploid,Cells, Haploid,Haploid Cells,Haploidies,Haploids
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012333 RNA, Messenger RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm. Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated
D013087 Spermatids Male germ cells derived from the haploid secondary SPERMATOCYTES. Without further division, spermatids undergo structural changes and give rise to SPERMATOZOA. Spermatoblasts,Spermatid,Spermatoblast
D014212 Tretinoin An important regulator of GENE EXPRESSION during growth and development, and in NEOPLASMS. Tretinoin, also known as retinoic acid and derived from maternal VITAMIN A, is essential for normal GROWTH; and EMBRYONIC DEVELOPMENT. An excess of tretinoin can be teratogenic. It is used in the treatment of PSORIASIS; ACNE VULGARIS; and several other SKIN DISEASES. It has also been approved for use in promyelocytic leukemia (LEUKEMIA, PROMYELOCYTIC, ACUTE). Retinoic Acid,Vitamin A Acid,Retin-A,Tretinoin Potassium Salt,Tretinoin Sodium Salt,Tretinoin Zinc Salt,Vesanoid,all-trans-Retinoic Acid,beta-all-trans-Retinoic Acid,trans-Retinoic Acid,Acid, Retinoic,Acid, Vitamin A,Acid, all-trans-Retinoic,Acid, beta-all-trans-Retinoic,Acid, trans-Retinoic,Potassium Salt, Tretinoin,Retin A,Salt, Tretinoin Potassium,Salt, Tretinoin Sodium,Salt, Tretinoin Zinc,Sodium Salt, Tretinoin,Zinc Salt, Tretinoin,all trans Retinoic Acid,beta all trans Retinoic Acid,trans Retinoic Acid

Related Publications

Meng Ji, and Shuang Tang, and Wenhua Pei, and Mingming Ning, and Yuehui Ma, and Xiangchen Li, and Weijun Guan
From primordial germ cells to spermatids in Caenorhabditis elegans.
July 2022, Seminars in cell & developmental biology,
Meng Ji, and Shuang Tang, and Wenhua Pei, and Mingming Ning, and Yuehui Ma, and Xiangchen Li, and Weijun Guan
Generation of Genome-Edited Chicken Through Targeting of Primordial Germ Cells.
January 2023, Methods in molecular biology (Clifton, N.J.),
Meng Ji, and Shuang Tang, and Wenhua Pei, and Mingming Ning, and Yuehui Ma, and Xiangchen Li, and Weijun Guan
Generation of functional oocytes and spermatids from fetal primordial germ cells after ectopic transplantation in adult mice.
April 2011, Biology of reproduction,
Meng Ji, and Shuang Tang, and Wenhua Pei, and Mingming Ning, and Yuehui Ma, and Xiangchen Li, and Weijun Guan
Analysis of chicken primordial germ cells.
June 2008, Cytotechnology,
Meng Ji, and Shuang Tang, and Wenhua Pei, and Mingming Ning, and Yuehui Ma, and Xiangchen Li, and Weijun Guan
Generation of primordial germ cells from pluripotent stem cells.
January 2009, Differentiation; research in biological diversity,
Meng Ji, and Shuang Tang, and Wenhua Pei, and Mingming Ning, and Yuehui Ma, and Xiangchen Li, and Weijun Guan
Uniparental chicken offsprings derived from oogenesis of chicken primordial germ cells (ZZ).
March 2017, Biology of reproduction,
Meng Ji, and Shuang Tang, and Wenhua Pei, and Mingming Ning, and Yuehui Ma, and Xiangchen Li, and Weijun Guan
Enhanced cultivation of chicken primordial germ cells.
July 2023, Scientific reports,
Meng Ji, and Shuang Tang, and Wenhua Pei, and Mingming Ning, and Yuehui Ma, and Xiangchen Li, and Weijun Guan
Generation of viable fish from cryopreserved primordial germ cells.
February 2007, Molecular reproduction and development,
Meng Ji, and Shuang Tang, and Wenhua Pei, and Mingming Ning, and Yuehui Ma, and Xiangchen Li, and Weijun Guan
Cholesterol induces proliferation of chicken primordial germ cells.
August 2016, Animal reproduction science,
Meng Ji, and Shuang Tang, and Wenhua Pei, and Mingming Ning, and Yuehui Ma, and Xiangchen Li, and Weijun Guan
A method for cryopreserving chicken primordial germ cells.
October 2006, Poultry science,
Copied contents to your clipboard!