Biomaterial Database

Behavior of the IncW plasmid Sa in Zymomonas mobilis. 1987

A T Strzelecki, and A E Goodman, and P L Rogers

Department of Biotechnology, University of New South Wales, Australia.

The stability of the broad-host-range IncW R plasmid Sa in Zymomonas mobilis ZM6100(Sa) was monitored using three antibiotic resistance markers carried by Sa. When grown in batch culture without selection, ZM6100(Sa) rapidly lost the Sa plasmid. When grown with selection for either kanamycin or spectinomycin resistance, the three Sa markers were retained in at least 90% of the population, with spontaneous loss of chloramphenicol resistance being observed in the rest of the population. When ZM6100(Sa) was grown with selection for chloramphenicol resistance, the Sa plasmid either formed a cointegrate plasmid, designated pNSW301, with a native ZM6100 plasmid or was lost except for the chloramphenicol resistance marker. Possible transportation of the chloramphenicol resistance region of Sa to the Z. mobilis chromosome was indicated by hybridization data. The cointegrate plasmid, pNSW301, was maintained stably, except for spontaneous loss of chloramphenicol resistance, in Z. mobilis without selection for 300 generations. pNSW301 was able to replicate in Escherichia coli and was capable of conjugal transfer.

UI	MeSH Term	Description	Entries
D007613	Kanamycin Resistance	Nonsusceptibility of bacteria to the antibiotic KANAMYCIN, which can bind to their 70S ribosomes and cause misreading of messenger RNA.
D011815	R Factors	A class of plasmids that transfer antibiotic resistance from one bacterium to another by conjugation.	R Factor,R Plasmid,R Plasmids,Resistance Factor,Resistance Factors,Factor, R,Factor, Resistance,Factors, R,Factors, Resistance,Plasmid, R,Plasmids, R
D002702	Chloramphenicol Resistance	Nonsusceptibility of bacteria to the action of CHLORAMPHENICOL, a potent inhibitor of protein synthesis in the 50S ribosomal subunit where amino acids are added to nascent bacterial polypeptides.	Chloramphenicol Resistances
D003227	Conjugation, Genetic	A parasexual process in BACTERIA; ALGAE; FUNGI; and ciliate EUKARYOTA for achieving exchange of chromosome material during fusion of two cells. In bacteria, this is a uni-directional transfer of genetic material; in protozoa it is a bi-directional exchange. In algae and fungi, it is a form of sexual reproduction, with the union of male and female gametes.	Bacterial Conjugation,Conjugation, Bacterial,Genetic Conjugation
D004352	Drug Resistance, Microbial	The ability of microorganisms, especially bacteria, to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).	Antibiotic Resistance,Antibiotic Resistance, Microbial,Antimicrobial Resistance, Drug,Antimicrobial Drug Resistance,Antimicrobial Drug Resistances,Antimicrobial Resistances, Drug,Drug Antimicrobial Resistance,Drug Antimicrobial Resistances,Drug Resistances, Microbial,Resistance, Antibiotic,Resistance, Drug Antimicrobial,Resistances, Drug Antimicrobial
D004926	Escherichia coli	A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.	Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D006090	Gram-Negative Bacteria	Bacteria which lose crystal violet stain but are stained pink when treated by Gram's method.	Gram Negative Bacteria
D000198	Spectinomycin	An antibiotic produced by Streptomyces spectabilis. It is active against gram-negative bacteria and used for the treatment of GONORRHEA.	Actinospectacin,Adspec,Ferkel Spectam,Kempi,Prospec,Salmosan-T,Spectam,Spectinomycin Dihydrochloride, Anhydrous,Spectinomycin Dihydrochloride, Pentahydrate,Spectinomycin Hydrochloride,Stanilo,Trobicin,Salmosan T,Spectam, Ferkel