Characterization of a conjunctival melanoma cell line CM-AS16, newly-established from a metastatic Han Chinese patient. 2018

Yongyun Li, and Qingfeng Shang, and Peng Li, and Jinfeng Cao, and Liqi Zhu, and Martine J Jager, and Xianqun Fan, and Shengfang Ge, and Renbing Jia
Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, No. 12, Lane 833, Zhizaoju Road, Huangpu District, Shanghai, 200001, China; Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, No. 12, Lane 833, Zhizaoju Road, Huangpu District, Shanghai, 200001, China. Electronic address: liyongyun@sjtu.edu.cn.

Conjunctival melanoma (CM) is associated with metastases formation, can be fatal, and occurs in all different races. While cell lines are essential for experimental research, all available CM cell lines are derived from Caucasian patients. Furthermore, they are not derived from metastases. We aimed to establish a new CM cell line from a parotid metastasis in a Han Chinese patient and to depict its characteristics. The novel cell line, CM-AS16, was obtained from a surgical parotid sample and determined as a unique one with short tandem repeat (STR) analysis. It has been successively sub-cultured in vitro for more than 100 passages and exhibits rapid proliferation and migration. Chromosome analysis shows abundant chromosome aberrations, while whole exome sequencing (WES) reveals a typical NRAS mutation (Q61R). In vivo tumor growth was successfully established in a NOD/SCID mice model, and the immunophenotypes, such as HMB45, Melan A, S100, SOX10 and Ki67, manifested similar between the original tumor and the xenograft by immunohistochemistry. A MEK inhibitor binimetinib prominently suppressed in vitro cell growth by inhibiting ERK1/2 phosphorylation. In addition, monoclonal cells were used to demonstrate the drug sensitivity of different cells. In conclusion, the first cell line, CM-AS16, that is derived from a CM in a Han Chinese patient has highly malignant characteristics and a typical NRAS mutation. It may be used as a tool for further exploration of the molecular mechanisms of CM.

UI MeSH Term Description Entries
D008207 Lymphatic Metastasis Transfer of a neoplasm from its primary site to lymph nodes or to distant parts of the body by way of the lymphatic system. Lymph Node Metastasis,Lymph Node Metastases,Lymphatic Metastases,Metastasis, Lymph Node
D008297 Male Males
D008545 Melanoma A malignant neoplasm derived from cells that are capable of forming melanin, which may occur in the skin of any part of the body, in the eye, or, rarely, in the mucous membranes of the genitalia, anus, oral cavity, or other sites. It occurs mostly in adults and may originate de novo or from a pigmented nevus or malignant lentigo. Melanomas frequently metastasize widely, and the regional lymph nodes, liver, lungs, and brain are likely to be involved. The incidence of malignant skin melanomas is rising rapidly in all parts of the world. (Stedman, 25th ed; from Rook et al., Textbook of Dermatology, 4th ed, p2445) Malignant Melanoma,Malignant Melanomas,Melanoma, Malignant,Melanomas,Melanomas, Malignant
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D009363 Neoplasm Proteins Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm. Proteins, Neoplasm
D010306 Parotid Gland The largest of the three pairs of SALIVARY GLANDS. They lie on the sides of the FACE immediately below and in front of the EAR. Gland, Parotid,Glands, Parotid,Parotid Glands
D011905 Genes, ras Family of retrovirus-associated DNA sequences (ras) originally isolated from Harvey (H-ras, Ha-ras, rasH) and Kirsten (K-ras, Ki-ras, rasK) murine sarcoma viruses. Ras genes are widely conserved among animal species and sequences corresponding to both H-ras and K-ras genes have been detected in human, avian, murine, and non-vertebrate genomes. The closely related N-ras gene has been detected in human neuroblastoma and sarcoma cell lines. All genes of the family have a similar exon-intron structure and each encodes a p21 protein. Ha-ras Genes,Ki-ras Genes,N-ras Genes,c-Ha-ras Genes,c-Ki-ras Genes,c-N-ras Genes,ras Genes,v-Ha-ras Genes,v-Ki-ras Genes,H-ras Genes,H-ras Oncogenes,Ha-ras Oncogenes,K-ras Genes,K-ras Oncogenes,Ki-ras Oncogenes,N-ras Oncogenes,c-H-ras Genes,c-H-ras Proto-Oncogenes,c-Ha-ras Proto-Oncogenes,c-K-ras Genes,c-K-ras Proto-Oncogenes,c-Ki-ras Proto-Oncogenes,c-N-ras Proto-Oncogenes,ras Oncogene,v-H-ras Genes,v-H-ras Oncogenes,v-Ha-ras Oncogenes,v-K-ras Genes,v-K-ras Oncogenes,v-Ki-ras Oncogenes,Gene, Ha-ras,Gene, Ki-ras,Gene, v-Ha-ras,Gene, v-Ki-ras,Genes, Ha-ras,Genes, Ki-ras,Genes, N-ras,Genes, v-Ha-ras,Genes, v-Ki-ras,H ras Genes,H ras Oncogenes,H-ras Gene,H-ras Oncogene,Ha ras Genes,Ha ras Oncogenes,Ha-ras Gene,Ha-ras Oncogene,K ras Genes,K ras Oncogenes,K-ras Gene,K-ras Oncogene,Ki ras Genes,Ki ras Oncogenes,Ki-ras Gene,Ki-ras Oncogene,N ras Genes,N ras Oncogenes,N-ras Gene,N-ras Oncogene,c H ras Genes,c H ras Proto Oncogenes,c Ha ras Genes,c Ha ras Proto Oncogenes,c K ras Genes,c K ras Proto Oncogenes,c Ki ras Genes,c Ki ras Proto Oncogenes,c N ras Genes,c N ras Proto Oncogenes,c-H-ras Gene,c-H-ras Proto-Oncogene,c-Ha-ras Gene,c-Ha-ras Proto-Oncogene,c-K-ras Gene,c-K-ras Proto-Oncogene,c-Ki-ras Gene,c-Ki-ras Proto-Oncogene,c-N-ras Gene,c-N-ras Proto-Oncogene,ras Gene,ras Oncogenes,v H ras Genes,v H ras Oncogenes,v Ha ras Genes,v Ha ras Oncogenes,v K ras Genes,v K ras Oncogenes,v Ki ras Genes,v Ki ras Oncogenes,v-H-ras Gene,v-H-ras Oncogene,v-Ha-ras Gene,v-Ha-ras Oncogene,v-K-ras Gene,v-K-ras Oncogene,v-Ki-ras Gene,v-Ki-ras Oncogene
D002453 Cell Cycle The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE. Cell Division Cycle,Cell Cycles,Cell Division Cycles,Cycle, Cell,Cycle, Cell Division,Cycles, Cell,Cycles, Cell Division,Division Cycle, Cell,Division Cycles, Cell
D002681 China A country spanning from central Asia to the Pacific Ocean. Inner Mongolia,Manchuria,People's Republic of China,Sinkiang,Mainland China
D003230 Conjunctival Neoplasms Tumors or cancer of the CONJUNCTIVA. Conjunctival Neoplasm,Neoplasm, Conjunctival,Neoplasms, Conjunctival

Related Publications

Yongyun Li, and Qingfeng Shang, and Peng Li, and Jinfeng Cao, and Liqi Zhu, and Martine J Jager, and Xianqun Fan, and Shengfang Ge, and Renbing Jia
January 2021, American journal of translational research,
Yongyun Li, and Qingfeng Shang, and Peng Li, and Jinfeng Cao, and Liqi Zhu, and Martine J Jager, and Xianqun Fan, and Shengfang Ge, and Renbing Jia
August 1989, International journal of cancer,
Yongyun Li, and Qingfeng Shang, and Peng Li, and Jinfeng Cao, and Liqi Zhu, and Martine J Jager, and Xianqun Fan, and Shengfang Ge, and Renbing Jia
January 2001, International journal of urology : official journal of the Japanese Urological Association,
Yongyun Li, and Qingfeng Shang, and Peng Li, and Jinfeng Cao, and Liqi Zhu, and Martine J Jager, and Xianqun Fan, and Shengfang Ge, and Renbing Jia
November 1994, In vitro cellular & developmental biology. Animal,
Yongyun Li, and Qingfeng Shang, and Peng Li, and Jinfeng Cao, and Liqi Zhu, and Martine J Jager, and Xianqun Fan, and Shengfang Ge, and Renbing Jia
July 1998, International journal of cancer,
Yongyun Li, and Qingfeng Shang, and Peng Li, and Jinfeng Cao, and Liqi Zhu, and Martine J Jager, and Xianqun Fan, and Shengfang Ge, and Renbing Jia
May 1987, Nihon Sanka Fujinka Gakkai zasshi,
Yongyun Li, and Qingfeng Shang, and Peng Li, and Jinfeng Cao, and Liqi Zhu, and Martine J Jager, and Xianqun Fan, and Shengfang Ge, and Renbing Jia
January 2010, Histochemistry and cell biology,
Yongyun Li, and Qingfeng Shang, and Peng Li, and Jinfeng Cao, and Liqi Zhu, and Martine J Jager, and Xianqun Fan, and Shengfang Ge, and Renbing Jia
June 1998, Virchows Archiv : an international journal of pathology,
Yongyun Li, and Qingfeng Shang, and Peng Li, and Jinfeng Cao, and Liqi Zhu, and Martine J Jager, and Xianqun Fan, and Shengfang Ge, and Renbing Jia
June 1992, Journal of the Formosan Medical Association = Taiwan yi zhi,
Yongyun Li, and Qingfeng Shang, and Peng Li, and Jinfeng Cao, and Liqi Zhu, and Martine J Jager, and Xianqun Fan, and Shengfang Ge, and Renbing Jia
June 1984, Nihon Sanka Fujinka Gakkai zasshi,
Copied contents to your clipboard!