-transparent.png){kind=logo}
The presence of biologically active atrial natriuretic factor (ANF)-like peptides was demonstrated in rat anterior pituitary. ANF-like immunoreactivity was detected in rat anterior pituitary by specific radioimmunoassay and was extracted from rat anterior pituitary homogenates by heat-activated Vycor glass beads; extracts were purified by reverse-phase high performance liquid chromatography. Two peaks containing ANF immunoreactive material were obtained. The first peak was eluted from the C18 mu Bondapak column at a position similar to the 28-amino acid carboxy terminal peptide (Ser99-Tyr126)-ANF of prohormone. The second peak had the same pattern of elution as the 126-amino acid prohormone, (Asn1-Tyr126)-ANF. The biological activity of the smaller molecular weight peptide (28 amino acid) was assessed by its inhibitory effect on 10(-8) M ACTH-stimulated aldosterone secretion in rat zona glomerulosa cell suspension. This ANF-like material also displaced I125-labelled ANF from rat glomerular receptors with a potency similar to synthetic (Arg101-Tyr126)-ANF. Immunocytochemical localization revealed a distribution of ANF-stained cells similar in pattern and location to that of gonadotrophs. These results suggest the existence of biologically active ANF-like peptides and ANF prohormone within the anterior pituitary. However, their role remains to be elucidated.
J Gutkowska, and
M Cantin
June 1988,
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme,
J Gutkowska, and
M Cantin
February 1988,
Molecular and cellular endocrinology,
J Gutkowska, and
M Cantin
January 1987,
Proceedings of the National Academy of Sciences of the United States of America,
J Gutkowska, and
M Cantin
November 1985,
Biochemical and biophysical research communications,
J Gutkowska, and
M Cantin
March 1986,
Biochemical and biophysical research communications,
J Gutkowska, and
M Cantin
July 1992,
The Clinical investigator,
J Gutkowska, and
M Cantin
September 1988,
Kidney international. Supplement,
J Gutkowska, and
M Cantin
October 1986,
Regulatory peptides,
J Gutkowska, and
M Cantin
June 2000,
Regulatory peptides,
