BIOMAT Database Logo BIOMAT Database Logo

Identification of Glucose-Regulated Protein 78 (GRP78) as a Receptor in BHK-21 Cells for Duck Tembusu Virus Infection. 2018

Dongmin Zhao, and Qingtao Liu, and Kaikai Han, and Huili Wang, and Jing Yang, and Keran Bi, and Yuzhuo Liu, and Na Liu, and Yujie Tian, and Yin Li
Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, China.

Since 2010, outbreak and spread of tembusu virus (TMUV) caused huge losses to the breeding industry of waterfowl in several provinces of China. In this study, we identify the glucose-regulated protein 78 (GRP78) as a receptor in BHK-21 cells for duck TMUV infection. Using cell membrane from BHK-21 cells, a TMUV-binding protein of approximately 70 kDa was observed by viral overlay protein binding assay (VOPBA). LC-MS/MS analysis and co-immunoprecipitation identified GRP78 as a protein interacting with TMUV. Antibody against GRP78 inhibited the binding of TMUV to the cell surface of BHK-21 cells. Indirect immunofluorescence studies showed the colocalization of GRP78 with TMUV in virus-infected BHK-21 cells. We found that GRP78 over-expression increased TMUV infection, whereas GRP78 knockdown by using a specific small interfering RNA inhibited TMUV infection in BHK-21 cells. Taken together, our results indicate that GRP78 is a novel host factor involved in TMUV entry.

UI MeSH Term Description Entries

Related Publications

Dongmin Zhao, and Qingtao Liu, and Kaikai Han, and Huili Wang, and Jing Yang, and Keran Bi, and Yuzhuo Liu, and Na Liu, and Yujie Tian, and Yin Li
Proteome analysis of Duck Tembusu virus (DTMUV)-infected BHK-21 cells.
June 2017, Proteomics,
Dongmin Zhao, and Qingtao Liu, and Kaikai Han, and Huili Wang, and Jing Yang, and Keran Bi, and Yuzhuo Liu, and Na Liu, and Yujie Tian, and Yin Li
[Duck viperin can express in transfected BHK-21 cells and inhibit germination of Duck Tembusu virus].
May 2019, Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology,
Dongmin Zhao, and Qingtao Liu, and Kaikai Han, and Huili Wang, and Jing Yang, and Keran Bi, and Yuzhuo Liu, and Na Liu, and Yujie Tian, and Yin Li
Comparison of endocytosis pathways of Duck Tembusu virus in BHK-21 and duck embryo fibroblasts.
September 2023, Poultry science,
Dongmin Zhao, and Qingtao Liu, and Kaikai Han, and Huili Wang, and Jing Yang, and Keran Bi, and Yuzhuo Liu, and Na Liu, and Yujie Tian, and Yin Li
Attenuation of duck Tembusu virus ZJSBL01 strain following serial passage in BHK-21 cells supplied with 5-Fluorouracil.
November 2019, Virus research,
Dongmin Zhao, and Qingtao Liu, and Kaikai Han, and Huili Wang, and Jing Yang, and Keran Bi, and Yuzhuo Liu, and Na Liu, and Yujie Tian, and Yin Li
Expression and release of glucose-regulated protein-78 (GRP78) in multiple myeloma.
August 2017, Oncotarget,
Dongmin Zhao, and Qingtao Liu, and Kaikai Han, and Huili Wang, and Jing Yang, and Keran Bi, and Yuzhuo Liu, and Na Liu, and Yujie Tian, and Yin Li
Cloning and expression of glucose regulated protein 78 (GRP78) in Fenneropenaeus chinensis.
February 2009, Molecular biology reports,
Dongmin Zhao, and Qingtao Liu, and Kaikai Han, and Huili Wang, and Jing Yang, and Keran Bi, and Yuzhuo Liu, and Na Liu, and Yujie Tian, and Yin Li
Glucose-regulated protein 78 (Grp78) confers chemoresistance to tumor endothelial cells under acidic stress.
January 2014, PloS one,
Dongmin Zhao, and Qingtao Liu, and Kaikai Han, and Huili Wang, and Jing Yang, and Keran Bi, and Yuzhuo Liu, and Na Liu, and Yujie Tian, and Yin Li
Long-term passage of duck Tembusu virus in BHK-21 cells generates a completely attenuated and immunogenic population with increased genetic diversity.
January 2020, Vaccine,
Dongmin Zhao, and Qingtao Liu, and Kaikai Han, and Huili Wang, and Jing Yang, and Keran Bi, and Yuzhuo Liu, and Na Liu, and Yujie Tian, and Yin Li
Glucose-regulated protein 78 (GRP78) as a potential novel biomarker and therapeutic target in multiple myeloma.
November 2020, Expert review of hematology,
Dongmin Zhao, and Qingtao Liu, and Kaikai Han, and Huili Wang, and Jing Yang, and Keran Bi, and Yuzhuo Liu, and Na Liu, and Yujie Tian, and Yin Li
Hypothermic treatment restores glucose regulated protein 78 (GRP78) expression in ischemic brain.
November 2001, Brain research. Molecular brain research,
Copied contents to your clipboard!