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A high affinity, calmodulin-sensitive (Ca2 + Mg2+)-ATPase was demonstrated in the plasma membrane preparation of three different osteosarcoma cell lines previously demonstrated to respond to parathyroid hormone with an increase in cytosolic calcium and a decrease in pH. The maximal velocity of the enzyme activity in the membrane preparations ranged from 0.83 to 2.42 nmol Pi released per min per mg protein with half-saturation constants of 26 nM of free Ca. The enzyme activity was not affected by Na+, K+, ouabain and azide, and exhibited an absolute requirement for Mg2+ ions. These results suggest a possible role for a membrane Ca2 + Mg2+-ATPase in initiating and perpetuating the ionic control of osteoblastic function.
V Shen, and
K Hruska, and
L V Avioli
June 1988,
Archives of biochemistry and biophysics,
V Shen, and
K Hruska, and
L V Avioli
October 1991,
Molecular and cellular biochemistry,
V Shen, and
K Hruska, and
L V Avioli
January 1988,
Progress in biophysics and molecular biology,
V Shen, and
K Hruska, and
L V Avioli
January 1984,
Biochimica et biophysica acta,
V Shen, and
K Hruska, and
L V Avioli
September 1982,
Archives of andrology,
V Shen, and
K Hruska, and
L V Avioli
December 2000,
Journal of gastroenterology,
V Shen, and
K Hruska, and
L V Avioli
June 1990,
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research,
V Shen, and
K Hruska, and
L V Avioli
January 2000,
Ukrains'kyi biokhimichnyi zhurnal (1999 ),
V Shen, and
K Hruska, and
L V Avioli
December 1985,
Biochimica et biophysica acta,
V Shen, and
K Hruska, and
L V Avioli
January 1981,
Acta biologica et medica Germanica,
