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An apparently macromolecular factor is isolated from aqueous skin extracts which inhibits DNA synthesis in vivo and in vitro with high efficacy (ID50 in vivo 0.2 pmol/g, in vitro 0.2 pM) and in a highly specific manner showing a point of attack in the late G1-phase of the cell cycle (epidermal G1-chalone). Preliminary characterization indicates an unusual highly amphipathic structure consisting of amino acids and carbohydrate. Despite its apparent molecular weight of approximately 10 kD the chalone is stable against denaturing agents and most enzymes, including proteases. An inverse correlation between chalone responsiveness of mouse epidermis in vivo and the development of hyperplasia due to injury indicates an important role of the factor in the regulation of tissue homeostasis. According to its physicochemical and biological properties the epidermal G1-chalone appears not to be related to other endogenous inhibitors of epidermal cell proliferation such as the pentapeptide pyroGlu-Glu-Asp-Ser-GlyOH and transforming growth factor beta (TGF beta).
K H Richter, and
M Clauss, and
W Höfle, and
R Schnapke, and
F Marks
September 1978,
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K H Richter, and
M Clauss, and
W Höfle, and
R Schnapke, and
F Marks
January 1971,
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K H Richter, and
M Clauss, and
W Höfle, and
R Schnapke, and
F Marks
December 1975,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
K H Richter, and
M Clauss, and
W Höfle, and
R Schnapke, and
F Marks
July 1975,
Experimental cell research,
K H Richter, and
M Clauss, and
W Höfle, and
R Schnapke, and
F Marks
January 1965,
Acta pathologica et microbiologica Scandinavica,
K H Richter, and
M Clauss, and
W Höfle, and
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F Marks
January 1988,
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K H Richter, and
M Clauss, and
W Höfle, and
R Schnapke, and
F Marks
May 1971,
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K H Richter, and
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F Marks
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K H Richter, and
M Clauss, and
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F Marks
March 1971,
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K H Richter, and
M Clauss, and
W Höfle, and
R Schnapke, and
F Marks
July 1968,
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