BIOMAT Database Logo BIOMAT Database Logo

Biofilm formation on different pH conditions by Streptococcus agalactiae isolated from bovine mastitic milk. 2018

P S D Miranda, and P S Lannes-Costa, and B A S Pimentel, and L G Silva, and B T Ferreira-Carvalho, and G C Menezes, and A L Mattos-Guaraldi, and R Hirata, and R A Mota, and P E Nagao
Laboratory of Molecular Biology and Physiology of Streptococci, Institute of Biology Roberto Alcantara Gomes, Rio de Janeiro State University, Rio de Janeiro, Brazil.

Streptococcus agalactiae is among the most relevant aetiologic agent of bovine clinical and subclinical mastitis, a major problem for the dairy industry. In Brazil, clonal diversity, capsular typing and multidrug resistance profiles of S. agalactiae related to human and bovine infections need further investigation. Presently, S. agalactiae isolates of bovine subclinical mastitis, from Brazilian Northeastern region, were submitted to capsular and pulsed-field gel electrophoresis (PFGE)-typing, antimicrobial susceptibility and assays of biofilm formation at different time incubation and pH levels. Sixteen bovine isolates were characterized by polymerase chain reaction assay as S. agalactiae capsular type II (CTII) and classified by PFGE in A1/A2 (n = 06), B1/B2 (n = 06), C (n = 03) and D (n = 01) patterns. Bovine S. agalactiae CTII strains were classified as 25% multidrug-resistant (MDR) with susceptibility to penicillin, linezolid and vancomycin. Biofilm formation on abiotic surface was strain- and time-dependent with significantly higher rates at pH 6·5. In conclusion, S. agalactiae capsular type II isolates recovered from bovine subclinical mastitis produced different pH-dependent biofilm levels. Our findings suggest that biofilm production is modulated by environmental factors and provides S. agalactiae advantageous in colonizing mammary gland during mastitis development, including MDR strains. CONCLUSIONS Streptococcus agalactiae is among the most relevant aetiologic agent of bovine clinical and subclinical mastitis, a major problem for the dairy industry. The disease may cause significant economic loss due to decreased production and milk quality and increased use of medicaments. Presently, data demonstrated that biofilm formation favours the establishment of infectious process in health mammary tissue by S. agalactiae and emphasizes that an acidic pH promotes adhesion by biofilm-forming bacterial strains. S. agalactiae strains (25%) showed resistance to tetracycline, azithromycin, erythromycin and clindamycin, and consequently were classified as multidrug-resistant strains.

UI MeSH Term Description Entries
D008414 Mastitis, Bovine INFLAMMATION of the UDDER in cows. Bovine Mastitides,Bovine Mastitis,Mastitides, Bovine
D008892 Milk The off-white liquid secreted by the mammary glands of humans and other mammals. It contains proteins, sugar, lipids, vitamins, and minerals. Cow Milk,Cow's Milk,Milk, Cow,Milk, Cow's
D001938 Brazil A country located on the eastern coast of South America, located between Colombia and Peru, that borders the Atlantic Ocean. It is bordered on the north by Venezuela, Guyana, Suriname, and French Guiana, on the south by Uruguay, and on the west by Argentina. The capital is Brasilia.
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D005260 Female Females
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000900 Anti-Bacterial Agents Substances that inhibit the growth or reproduction of BACTERIA. Anti-Bacterial Agent,Anti-Bacterial Compound,Anti-Mycobacterial Agent,Antibacterial Agent,Antibiotics,Antimycobacterial Agent,Bacteriocidal Agent,Bacteriocide,Anti-Bacterial Compounds,Anti-Mycobacterial Agents,Antibacterial Agents,Antibiotic,Antimycobacterial Agents,Bacteriocidal Agents,Bacteriocides,Agent, Anti-Bacterial,Agent, Anti-Mycobacterial,Agent, Antibacterial,Agent, Antimycobacterial,Agent, Bacteriocidal,Agents, Anti-Bacterial,Agents, Anti-Mycobacterial,Agents, Antibacterial,Agents, Antimycobacterial,Agents, Bacteriocidal,Anti Bacterial Agent,Anti Bacterial Agents,Anti Bacterial Compound,Anti Bacterial Compounds,Anti Mycobacterial Agent,Anti Mycobacterial Agents,Compound, Anti-Bacterial,Compounds, Anti-Bacterial
D013292 Streptococcus agalactiae A bacterium which causes mastitis in cattle and occasionally in man. Streptococcus Group B
D018441 Biofilms Encrustations formed from microbes (bacteria, algae, fungi, plankton, or protozoa) embedded in an EXTRACELLULAR POLYMERIC SUBSTANCE MATRIX that is secreted by the microbes. They occur on body surfaces such as teeth (DENTAL DEPOSITS); inanimate objects, and bodies of water. Biofilms are prevented from forming by treating surfaces with DENTIFRICES; DISINFECTANTS; ANTI-INFECTIVE AGENTS; and anti-fouling agents. Biofilm
D024901 Drug Resistance, Multiple, Bacterial The ability of bacteria to resist or to become tolerant to several structurally and functionally distinct drugs simultaneously. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS). Drug Resistance, Extensive, Bacterial,Drug Resistance, Extensively, Bacterial,Extensive Antibacterial Drug Resistance,Extensively Antibacterial Drug Resistance,Multidrug Resistance, Bacterial,Multiple Antibacterial Drug Resistance,Bacterial Multidrug Resistance,Bacterial Multidrug Resistances,Resistance, Bacterial Multidrug

Related Publications

P S D Miranda, and P S Lannes-Costa, and B A S Pimentel, and L G Silva, and B T Ferreira-Carvalho, and G C Menezes, and A L Mattos-Guaraldi, and R Hirata, and R A Mota, and P E Nagao
Biofilm production under different atmospheres and growth media by Streptococcus agalactiae isolated from milk of cows with subclinical mastitis.
January 2020, Archives of microbiology,
P S D Miranda, and P S Lannes-Costa, and B A S Pimentel, and L G Silva, and B T Ferreira-Carvalho, and G C Menezes, and A L Mattos-Guaraldi, and R Hirata, and R A Mota, and P E Nagao
Anti-Biofilm Effect of Tea Saponin on a Streptococcus agalactiae Strain Isolated from Bovine Mastitis.
September 2020, Animals : an open access journal from MDPI,
P S D Miranda, and P S Lannes-Costa, and B A S Pimentel, and L G Silva, and B T Ferreira-Carvalho, and G C Menezes, and A L Mattos-Guaraldi, and R Hirata, and R A Mota, and P E Nagao
Biofilm formation by Streptococcus agalactiae: influence of environmental conditions and implicated virulence factors.
January 2015, Frontiers in cellular and infection microbiology,
P S D Miranda, and P S Lannes-Costa, and B A S Pimentel, and L G Silva, and B T Ferreira-Carvalho, and G C Menezes, and A L Mattos-Guaraldi, and R Hirata, and R A Mota, and P E Nagao
Recovery of Mycoplasma agalactiae from mastitic goat milk.
September 1983, Journal of the American Veterinary Medical Association,
P S D Miranda, and P S Lannes-Costa, and B A S Pimentel, and L G Silva, and B T Ferreira-Carvalho, and G C Menezes, and A L Mattos-Guaraldi, and R Hirata, and R A Mota, and P E Nagao
Studies on Streptococcus agalactiae isolated from bovine mastitis in Indonesia.
May 2002, Journal of veterinary medicine. B, Infectious diseases and veterinary public health,
P S D Miranda, and P S Lannes-Costa, and B A S Pimentel, and L G Silva, and B T Ferreira-Carvalho, and G C Menezes, and A L Mattos-Guaraldi, and R Hirata, and R A Mota, and P E Nagao
Protein degradation in bovine milk caused by Streptococcus agalactiae.
August 2012, The Journal of dairy research,
P S D Miranda, and P S Lannes-Costa, and B A S Pimentel, and L G Silva, and B T Ferreira-Carvalho, and G C Menezes, and A L Mattos-Guaraldi, and R Hirata, and R A Mota, and P E Nagao
Biovars of coagulase-positive staphylococci isolated from bovine mastitic milk.
June 1990, Microbiologia (Madrid, Spain),
P S D Miranda, and P S Lannes-Costa, and B A S Pimentel, and L G Silva, and B T Ferreira-Carvalho, and G C Menezes, and A L Mattos-Guaraldi, and R Hirata, and R A Mota, and P E Nagao
Susceptibilities against bovine lactoferrin with microorganisms isolated from mastitic milk.
October 2004, The Journal of veterinary medical science,
P S D Miranda, and P S Lannes-Costa, and B A S Pimentel, and L G Silva, and B T Ferreira-Carvalho, and G C Menezes, and A L Mattos-Guaraldi, and R Hirata, and R A Mota, and P E Nagao
Specific agglutination of Streptococcus agalactiae from bovine mastitis by casein components of bovine milk.
October 1984, Journal of dairy science,
P S D Miranda, and P S Lannes-Costa, and B A S Pimentel, and L G Silva, and B T Ferreira-Carvalho, and G C Menezes, and A L Mattos-Guaraldi, and R Hirata, and R A Mota, and P E Nagao
Molecular characterization of Streptococcus agalactiae and Streptococcus uberis isolates from bovine milk.
December 2012, Tropical animal health and production,
Copied contents to your clipboard!