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The Epstein-Barr virus DNA polymerase was purified from extracts of P3HR-1 cells treated with n-butyrate for induction of the viral cycle. Sequential chromatography on DNA cellulose, phosphocellulose, and blue Sepharose yielded an enzyme preparation purified more than 1,300-fold. The purified enzyme was distinct from cellular enzymes but resembled the viral DNA polymerase in cells infected with herpes simplex virus type 1 or 2. The active enzyme had an apparent molecular weight of 185,000 as estimated by gel filtration on Sephacryl S-300. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed a major polypeptide corresponding to a molecular weight of ca. 110,000. This polypeptide correlated with the catalytic function of the purified enzyme, whereas the other, less abundant polypeptides did not. By immunoblotting, the 110,000-molecular-weight polypeptide could be identified as a viral polypeptide. It could not be determined whether the native enzyme was composed of more than one polypeptide.
B Kallin, and
L Sternås, and
A K Saemundssen, and
J Luka, and
H Jörnvall, and
B Eriksson, and
P Z Tao, and
M T Nilsson, and
G Klein
January 1978,
IARC scientific publications,
B Kallin, and
L Sternås, and
A K Saemundssen, and
J Luka, and
H Jörnvall, and
B Eriksson, and
P Z Tao, and
M T Nilsson, and
G Klein
July 1984,
Journal of virology,
B Kallin, and
L Sternås, and
A K Saemundssen, and
J Luka, and
H Jörnvall, and
B Eriksson, and
P Z Tao, and
M T Nilsson, and
G Klein
September 1974,
Applied microbiology,
B Kallin, and
L Sternås, and
A K Saemundssen, and
J Luka, and
H Jörnvall, and
B Eriksson, and
P Z Tao, and
M T Nilsson, and
G Klein
January 1991,
Intervirology,
B Kallin, and
L Sternås, and
A K Saemundssen, and
J Luka, and
H Jörnvall, and
B Eriksson, and
P Z Tao, and
M T Nilsson, and
G Klein
January 1982,
Virology,
B Kallin, and
L Sternås, and
A K Saemundssen, and
J Luka, and
H Jörnvall, and
B Eriksson, and
P Z Tao, and
M T Nilsson, and
G Klein
March 1977,
International journal of cancer,
B Kallin, and
L Sternås, and
A K Saemundssen, and
J Luka, and
H Jörnvall, and
B Eriksson, and
P Z Tao, and
M T Nilsson, and
G Klein
September 1982,
Journal of virology,
B Kallin, and
L Sternås, and
A K Saemundssen, and
J Luka, and
H Jörnvall, and
B Eriksson, and
P Z Tao, and
M T Nilsson, and
G Klein
April 1983,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
B Kallin, and
L Sternås, and
A K Saemundssen, and
J Luka, and
H Jörnvall, and
B Eriksson, and
P Z Tao, and
M T Nilsson, and
G Klein
December 1994,
Uirusu,
B Kallin, and
L Sternås, and
A K Saemundssen, and
J Luka, and
H Jörnvall, and
B Eriksson, and
P Z Tao, and
M T Nilsson, and
G Klein
May 1985,
The Journal of general virology,
