Investigation of the Persistence of Penicillin G and Dihydrostreptomycin Residues in Milk of Lactating Buffaloes ( Bubalus bubalis) Using Ultra-High-Performance Liquid Chromatography and Tandem Mass Spectrometry. 2018

Claudia Chirollo, and Antonio Vassallo, and Fabrizio Dal Piaz, and Barbara Lamagna, and Gennaro Tortora, and Gianluca Neglia, and Nunziatina De Tommasi, and Lorella Severino
Department of Veterinary Medicine and Animal Production , University of Napoli "Federico II" , Via Delpino 1 , 80137 Napoli , Italy.

The purpose of this research was to evaluate the persistence of penicillin G and dihydrostreptomycin in milk of lactating buffaloes following intramuscular injection of procaine penicillin G (200000 IU/mL) and dihydrostreptomycin sulfate (250 mg/mL) every 24 h for 3 days. Milk samples were collected twice daily up to the 13th milking post-treatment and analyzed by ultra-high-performance liquid chromatography coupled to tandem mass spectrometry. The analytical method has been validated according to Commission Decision 2002/657/EC. The highest concentrations of penicillin G (275 μg kg-1) and dihydrostreptomycin (220.5 μg kg-1) were detected in the milk of the first milkings post-treatment, and levels were below the maximum residue limit of 4 and 200 μg kg-1 in all treated buffaloes at milkings 12 and 2, respectively. The results of this study demonstrate that a nine-milking withdrawal time set for bovine milk was not adequate for depletion of penicillin G in lactating buffaloes.

UI MeSH Term Description Entries
D008892 Milk The off-white liquid secreted by the mammary glands of humans and other mammals. It contains proteins, sugar, lipids, vitamins, and minerals. Cow Milk,Cow's Milk,Milk, Cow,Milk, Cow's
D010400 Penicillin G A penicillin derivative commonly used in the form of its sodium or potassium salts in the treatment of a variety of infections. It is effective against most gram-positive bacteria and against gram-negative cocci. It has also been used as an experimental convulsant because of its actions on GAMMA-AMINOBUTYRIC ACID mediated synaptic transmission. Benzylpenicillin,Benpen,Benzylpenicillin Potassium,Coliriocilina,Crystapen,Or-pen,Parcillin,Pekamin,Pengesod,Penibiot,Penicilina G Llorente,Penicillin G Jenapharm,Penicillin G Potassium,Penicillin G Sodium,Penicillin Grünenthal,Penilevel,Peniroger,Pfizerpen,Sodiopen,Sodipen,Sodium Benzylpenicillin,Sodium Penicillin,Unicilina,Ursopen,Van-Pen-G
D002020 Buffaloes Ruminants of the family Bovidae consisting of Bubalus arnee and Syncerus caffer. This concept is differentiated from BISON, which refers to Bison bison and Bison bonasus. Bubalus,Syncerus,Water Buffaloes,Buffalo,Water Buffalo,Buffalo, Water
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D004096 Dihydrostreptomycin Sulfate A semi-synthetic aminoglycoside antibiotic that is used in the treatment of TUBERCULOSIS. Dihydrostreptomycin,Citrocil,Sulfate, Dihydrostreptomycin
D004350 Drug Residues Drugs and their metabolites which are found in the edible tissues and milk of animals after their medication with specific drugs. This term can also apply to drugs found in adipose tissue of humans after drug treatment. Drug Residue,Residue, Drug,Residues, Drug
D005260 Female Females
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000900 Anti-Bacterial Agents Substances that inhibit the growth or reproduction of BACTERIA. Anti-Bacterial Agent,Anti-Bacterial Compound,Anti-Mycobacterial Agent,Antibacterial Agent,Antibiotics,Antimycobacterial Agent,Bacteriocidal Agent,Bacteriocide,Anti-Bacterial Compounds,Anti-Mycobacterial Agents,Antibacterial Agents,Antibiotic,Antimycobacterial Agents,Bacteriocidal Agents,Bacteriocides,Agent, Anti-Bacterial,Agent, Anti-Mycobacterial,Agent, Antibacterial,Agent, Antimycobacterial,Agent, Bacteriocidal,Agents, Anti-Bacterial,Agents, Anti-Mycobacterial,Agents, Antibacterial,Agents, Antimycobacterial,Agents, Bacteriocidal,Anti Bacterial Agent,Anti Bacterial Agents,Anti Bacterial Compound,Anti Bacterial Compounds,Anti Mycobacterial Agent,Anti Mycobacterial Agents,Compound, Anti-Bacterial,Compounds, Anti-Bacterial
D053719 Tandem Mass Spectrometry A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection. Mass Spectrometry-Mass Spectrometry,Mass Spectrometry Mass Spectrometry,Mass Spectrometry, Tandem

Related Publications

Claudia Chirollo, and Antonio Vassallo, and Fabrizio Dal Piaz, and Barbara Lamagna, and Gennaro Tortora, and Gianluca Neglia, and Nunziatina De Tommasi, and Lorella Severino
July 2011, Journal of agricultural and food chemistry,
Claudia Chirollo, and Antonio Vassallo, and Fabrizio Dal Piaz, and Barbara Lamagna, and Gennaro Tortora, and Gianluca Neglia, and Nunziatina De Tommasi, and Lorella Severino
November 2010, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
Claudia Chirollo, and Antonio Vassallo, and Fabrizio Dal Piaz, and Barbara Lamagna, and Gennaro Tortora, and Gianluca Neglia, and Nunziatina De Tommasi, and Lorella Severino
April 2018, Se pu = Chinese journal of chromatography,
Claudia Chirollo, and Antonio Vassallo, and Fabrizio Dal Piaz, and Barbara Lamagna, and Gennaro Tortora, and Gianluca Neglia, and Nunziatina De Tommasi, and Lorella Severino
March 2024, Journal of chromatography. A,
Claudia Chirollo, and Antonio Vassallo, and Fabrizio Dal Piaz, and Barbara Lamagna, and Gennaro Tortora, and Gianluca Neglia, and Nunziatina De Tommasi, and Lorella Severino
July 2010, Journal of chromatography. A,
Claudia Chirollo, and Antonio Vassallo, and Fabrizio Dal Piaz, and Barbara Lamagna, and Gennaro Tortora, and Gianluca Neglia, and Nunziatina De Tommasi, and Lorella Severino
March 2012, Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences,
Claudia Chirollo, and Antonio Vassallo, and Fabrizio Dal Piaz, and Barbara Lamagna, and Gennaro Tortora, and Gianluca Neglia, and Nunziatina De Tommasi, and Lorella Severino
February 2009, Journal of pharmaceutical and biomedical analysis,
Claudia Chirollo, and Antonio Vassallo, and Fabrizio Dal Piaz, and Barbara Lamagna, and Gennaro Tortora, and Gianluca Neglia, and Nunziatina De Tommasi, and Lorella Severino
January 2007, Rapid communications in mass spectrometry : RCM,
Claudia Chirollo, and Antonio Vassallo, and Fabrizio Dal Piaz, and Barbara Lamagna, and Gennaro Tortora, and Gianluca Neglia, and Nunziatina De Tommasi, and Lorella Severino
May 2012, Se pu = Chinese journal of chromatography,
Claudia Chirollo, and Antonio Vassallo, and Fabrizio Dal Piaz, and Barbara Lamagna, and Gennaro Tortora, and Gianluca Neglia, and Nunziatina De Tommasi, and Lorella Severino
March 2011, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
Copied contents to your clipboard!