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A human monoclonal antibody with anti-A specificity was generated by Epstein-Barr virus transformation of lymphocytes isolated from splenic tissue after in vitro stimulation with group A red blood cells. This antibody is of the IgM class and directly agglutinates group A red blood cells. Type A1, A2, Aint, A3, AX, Aend and A5 cells were agglutinated by the reagent indicating a single determinant is shared by these A subgroups.
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