Hand dexterity is an important index to assess whether extravehicular activity (EVA) gloves are appropriately designed. Pressurized gloves and low temperature environments can both cause a decrease in hand dexterity. However, due to the difficulty in performing tests under extreme conditions, there has been no report on dexterity tests with gloves under pressure and low temperature. To fill this gap, we performed a dexterity test of EVA gloves with twelve male volunteers involved under the extreme conditions, which were created in the low-pressure simulation cabin with vaporized liquid nitrogen used to cool it down. A total of nine conditions were designed. Purdue pegboard test and nut fastening test were improved before being applied in a hand dexterity test. Completion times for both tests, finger temperatures and cold feeling of the hand were recorded and analyzed. Results showed that the completion times for both tests increased either as the temperature decreased or as the pressure increased. Furthermore, a combined effect of low temperature and pressure was observed. The study provides evidence in support of astronaut training and optimization of EVA glove productivity.