-transparent.png){kind=logo}
The use of an affinity chromatography step performed with an immunoadsorbent consisting of anti-horse muscle acylphosphatase antibodies covalently linked to Sepharose 4B allowed us to purify horse heart acylphosphatase in a very rapid and efficient fashion. As in skeletal muscle, also in heart the enzyme is present as both a mixed disulfide with glutathione and a S-S dimer. The abundance of these forms in heart is quite lower than in skeletal muscle. The comparison of the molecular forms so purified with those obtained from horse skeletal muscle showed the same aminoacid composition, tryptic fingerprint, together with strictly similar apparent molecular weight and main kinetic parameters, supporting the conclusion that the acylphosphatase present in heart is the same enzyme as that purified from skeletal muscle.
M Stefani, and
G Camici, and
G Manao, and
G Cappugi, and
G Liguri, and
D Degl'Innocenti, and
N Landi, and
A Berti
January 1982,
Physiological chemistry and physics,
M Stefani, and
G Camici, and
G Manao, and
G Cappugi, and
G Liguri, and
D Degl'Innocenti, and
N Landi, and
A Berti
February 1991,
Journal of protein chemistry,
M Stefani, and
G Camici, and
G Manao, and
G Cappugi, and
G Liguri, and
D Degl'Innocenti, and
N Landi, and
A Berti
January 1976,
International journal of peptide and protein research,
M Stefani, and
G Camici, and
G Manao, and
G Cappugi, and
G Liguri, and
D Degl'Innocenti, and
N Landi, and
A Berti
December 1986,
Biochemistry,
M Stefani, and
G Camici, and
G Manao, and
G Cappugi, and
G Liguri, and
D Degl'Innocenti, and
N Landi, and
A Berti
November 1994,
European journal of biochemistry,
M Stefani, and
G Camici, and
G Manao, and
G Cappugi, and
G Liguri, and
D Degl'Innocenti, and
N Landi, and
A Berti
January 1993,
The International journal of biochemistry,
M Stefani, and
G Camici, and
G Manao, and
G Cappugi, and
G Liguri, and
D Degl'Innocenti, and
N Landi, and
A Berti
December 1987,
Journal of biochemistry,
M Stefani, and
G Camici, and
G Manao, and
G Cappugi, and
G Liguri, and
D Degl'Innocenti, and
N Landi, and
A Berti
January 1972,
The Journal of biological chemistry,
M Stefani, and
G Camici, and
G Manao, and
G Cappugi, and
G Liguri, and
D Degl'Innocenti, and
N Landi, and
A Berti
May 1988,
Biological chemistry Hoppe-Seyler,
M Stefani, and
G Camici, and
G Manao, and
G Cappugi, and
G Liguri, and
D Degl'Innocenti, and
N Landi, and
A Berti
October 1993,
Journal of protein chemistry,
