-transparent.png){kind=logo}
Enteric adenoviruses (EAds) (candidate adenoviruses 40 and 41, subgroups F and G) have been implicated in the etiology of gastroenteritis in infants, but their clinical significance has been unclear because a rapid test to distinguish these agents from other adenovirus (Ad) types has not been available. We developed a dot-blot hybridization assay for EAd DNA using a cloned DNA fragment that has little homology to non-EAd DNAs. The dot-blot system detected less than 20 pg of EAd DNA, while showing minimal cross hybridization to representative strains from all other Ad groups. There was no detectable hybridization to extracts of samples known to contain other enteric viruses. It was further shown that low levels of EAds in specimens could be amplified by culturing for 1 day in 293 cells. Stool samples and tissue culture lysates prescreened by electron microscopy, cell culture or ELISA were tested in a blind fashion. Using endonuclease analysis as the standard for typing the isolates, we found the dot-blot system to have a 91% sensitivity and 71% specificity for detecting EAds and distinguishing them from other Ads. False-positive and equivocal dot-blot results appeared to be caused by other Ads.
H E Takiff, and
M Seidlin, and
P Krause, and
J Rooney, and
C Brandt, and
W Rodriguez, and
R Yolken, and
S E Straus
February 1985,
Journal of medical virology,
H E Takiff, and
M Seidlin, and
P Krause, and
J Rooney, and
C Brandt, and
W Rodriguez, and
R Yolken, and
S E Straus
May 1991,
Journal of clinical microbiology,
H E Takiff, and
M Seidlin, and
P Krause, and
J Rooney, and
C Brandt, and
W Rodriguez, and
R Yolken, and
S E Straus
February 1992,
Epidemiology and infection,
H E Takiff, and
M Seidlin, and
P Krause, and
J Rooney, and
C Brandt, and
W Rodriguez, and
R Yolken, and
S E Straus
August 1995,
Molecular and cellular probes,
H E Takiff, and
M Seidlin, and
P Krause, and
J Rooney, and
C Brandt, and
W Rodriguez, and
R Yolken, and
S E Straus
February 1990,
Journal of virological methods,
H E Takiff, and
M Seidlin, and
P Krause, and
J Rooney, and
C Brandt, and
W Rodriguez, and
R Yolken, and
S E Straus
May 1984,
Virology,
H E Takiff, and
M Seidlin, and
P Krause, and
J Rooney, and
C Brandt, and
W Rodriguez, and
R Yolken, and
S E Straus
February 1985,
Journal of medical virology,
H E Takiff, and
M Seidlin, and
P Krause, and
J Rooney, and
C Brandt, and
W Rodriguez, and
R Yolken, and
S E Straus
July 1986,
Biochemical and biophysical research communications,
H E Takiff, and
M Seidlin, and
P Krause, and
J Rooney, and
C Brandt, and
W Rodriguez, and
R Yolken, and
S E Straus
November 1989,
Journal of virological methods,
H E Takiff, and
M Seidlin, and
P Krause, and
J Rooney, and
C Brandt, and
W Rodriguez, and
R Yolken, and
S E Straus
July 1992,
Journal of medical virology,
