The molecular basis for host responses to Marek's disease viruses integrated with different retro-viral long terminal repeat. 2018

Zhongwen Zhou, and Ning Cui, and Shuai Su, and Shuhong Sun, and Zhizhong Cui
College of Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong 271018, China.

Integration of retro-viral long terminal repeat (LTR) into the Marek's disease virus (MDV) genome can occur both in co-cultivation cell cultures and naturally in dual infected chickens. It is clear that the LTR insert is associated with the pathogenicity of MDV. The objective of this study was to compare the host responses to MDV with a different retro-viral LTR insert. Gene-chip containing chicken genome was employed to investigate the gene transcription profile of chicken embryo fibroblasts cells, and 795 genes were differentially expressed in chicken embryo fibroblasts infected with GX0101 with a reticuloendotheliosis virus LTR insert as compared to GX0101-ALV-LTR significantly. The differentially expressed genes were mostly associated with the regulation of transcription and the development of multiple organs. Based on the bio functions of the differential genes, infection of GX0101 was predicated with a greater development disorder of multiple systems, resulting in higher growth retardation, mortality, tumorigenicity, and immunosuppression in chickens than GX0101-ALV-LTR. Collectively, our results provided valuable insights into elucidation of the possible relationship between retro-viral LTR insert and the observed phenotypes caused by MDV recombinant viruses.

UI MeSH Term Description Entries
D008380 Marek Disease A transmissible viral disease of birds caused by avian herpesvirus 2 (HERPESVIRUS 2, GALLID) and other MARDIVIRUS. There is lymphoid cell infiltration or lymphomatous tumor formation in the peripheral nerves and gonads, but may also involve visceral organs, skin, muscle, and the eye. Fowl Paralysis,Marek's Disease,Fowl Paralyses,Mareks Disease,Paralyses, Fowl,Paralysis, Fowl
D008381 Herpesvirus 2, Gallid The type species of the genus MARDIVIRUS in the family HERPESVIRIDAE. It is the etiologic agent of MAREK DISEASE, infecting domestic fowl and wild birds. Fowl Paralysis Virus,Marek's Disease Herpesvirus 1,Marek's Disease Virus Serotype 1,Neurolymphomatosis Virus,Gallid Herpesvirus 2,Herpesvirus 2 (gamma), Gallid,Marek Disease Herpesvirus 1,Fowl Paralysis Viruses,Neurolymphomatosis Viruses,Paralysis Virus, Fowl,Paralysis Viruses, Fowl
D011201 Poultry Diseases Diseases of birds which are raised as a source of meat or eggs for human consumption and are usually found in barnyards, hatcheries, etc. The concept is differentiated from BIRD DISEASES which is for diseases of birds not considered poultry and usually found in zoos, parks, and the wild. Disease, Poultry,Diseases, Poultry,Poultry Disease
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D002642 Chick Embryo The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching. Embryo, Chick,Chick Embryos,Embryos, Chick
D005347 Fibroblasts Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules. Fibroblast
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013047 Specific Pathogen-Free Organisms Animals or humans raised in the absence of a particular disease-causing virus or other microorganism. Less frequently plants are cultivated pathogen-free. Pathogen-Free Organisms,Specific Pathogen Free,Organism, Pathogen-Free,Organism, Specific Pathogen-Free,Organisms, Pathogen-Free,Organisms, Specific Pathogen-Free,Pathogen Free Organisms,Pathogen Free, Specific,Pathogen Frees, Specific,Pathogen-Free Organism,Pathogen-Free Organism, Specific,Pathogen-Free Organisms, Specific,Specific Pathogen Free Organisms,Specific Pathogen-Free Organism
D059467 Transcriptome The pattern of GENE EXPRESSION at the level of genetic transcription in a specific organism or under specific circumstances in specific cells. Transcriptomes,Gene Expression Profiles,Gene Expression Signatures,Transcriptome Profiles,Expression Profile, Gene,Expression Profiles, Gene,Expression Signature, Gene,Expression Signatures, Gene,Gene Expression Profile,Gene Expression Signature,Profile, Gene Expression,Profile, Transcriptome,Profiles, Gene Expression,Profiles, Transcriptome,Signature, Gene Expression,Signatures, Gene Expression,Transcriptome Profile
D020079 Terminal Repeat Sequences Nucleotide sequences repeated on both the 5' and 3' ends of a sequence under consideration. For example, the hallmarks of a transposon are that it is flanked by inverted repeats on each end and the inverted repeats are flanked by direct repeats. The Delta element of Ty retrotransposons and LTRs (long terminal repeats) are examples of this concept. Delta Elements,Flanking Repeat Sequences,Inverted Terminal Repeat,Long Terminal Repeat,R Repetitive Sequence,Terminal Repeat,Delta Element,Element, Delta,Elements, Delta,Flanking Repeat Sequence,Long Terminal Repeats,R Repetitive Sequences,Repeat Sequence, Flanking,Repeat Sequence, Terminal,Repeat Sequences, Flanking,Repeat Sequences, Terminal,Repeat, Long Terminal,Repeat, Terminal,Repeats, Long Terminal,Repeats, Terminal,Repetitive Sequence, R,Repetitive Sequences, R,Sequence, Flanking Repeat,Sequence, R Repetitive,Sequence, Terminal Repeat,Sequences, Flanking Repeat,Sequences, R Repetitive,Sequences, Terminal Repeat,Terminal Repeat Sequence,Terminal Repeat, Long,Terminal Repeats,Terminal Repeats, Long

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