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K562 cells are induced by hemin to produce gamma and epsilon globin but not beta globin, although the beta globin gene is intact, and when isolated is expressed in a transient expression assay (1, 2). We have previously shown that an epsilon globin gene transferred into K562 cells is expressed and inducible (3). In this paper, we report the stable transfer of a sickle or betaS globin gene into K562 cells. Thirty-six different transformed lines were tested; 24 of 36 lines contained an intact betaS globin gene. However, using S1 nuclease, Dot blot, and Northern blotting analyses, none of these lines showed beta globin mRNA expression. These results indicate that trans acting factors are responsible for the lack of expression of the beta globin gene in K562 cells.
K Young, and
M Donovan-Peluso, and
R Cubbon, and
A Bank
January 1988,
Advances in experimental medicine and biology,
K Young, and
M Donovan-Peluso, and
R Cubbon, and
A Bank
January 1989,
Progress in clinical and biological research,
K Young, and
M Donovan-Peluso, and
R Cubbon, and
A Bank
January 1985,
Progress in clinical and biological research,
K Young, and
M Donovan-Peluso, and
R Cubbon, and
A Bank
January 1987,
Progress in clinical and biological research,
K Young, and
M Donovan-Peluso, and
R Cubbon, and
A Bank
December 1987,
The Journal of biological chemistry,
K Young, and
M Donovan-Peluso, and
R Cubbon, and
A Bank
January 1984,
Blood,
K Young, and
M Donovan-Peluso, and
R Cubbon, and
A Bank
May 1991,
Blood,
K Young, and
M Donovan-Peluso, and
R Cubbon, and
A Bank
June 1989,
Cell differentiation and development : the official journal of the International Society of Developmental Biologists,
K Young, and
M Donovan-Peluso, and
R Cubbon, and
A Bank
January 1989,
Cancer communications,
K Young, and
M Donovan-Peluso, and
R Cubbon, and
A Bank
July 1996,
The Journal of biological chemistry,
