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Rat renal medullary slices prelabeled with [14C]arachidonic acid generate [14C]diacylglycerol within 1 min of exposure to bradykinin action. Production of [14C]diacylglycerol is transient. 2 min after the addition of bradykinin, the levels of metabolite reach the maximum, but decrease thereafter. Simultaneously, bradykinin induces a parallel decrease of the radioactivity in phosphatidylinositol. No degradation of other phospholipids is observed, and triacylglycerol is not affected. The degradation of [14C]phosphatidylinositol to [14C]diacylglycerol indicated the presence of phospholipase C activity. Preincubation of prelabeled slices with 2 mM dibutyryl cyclic AMP prevents both the generation of diacylglycerol and the degradation of phosphatidylinositol. Neither mepacrine nor indomethacin block diacylglycerol production and phosphatidylinositol breakdown. We conclude that, when rat renal medullary slices are stimulated with bradykinin, phosphatidylinositol-specific phospholipase C is activated.
N Speziale, and
E H Speziale, and
J M Pasquini
October 1991,
Journal of the American Society of Nephrology : JASN,
N Speziale, and
E H Speziale, and
J M Pasquini
December 1994,
Neurochemistry international,
N Speziale, and
E H Speziale, and
J M Pasquini
January 1994,
Biochemical pharmacology,
N Speziale, and
E H Speziale, and
J M Pasquini
January 2014,
The Journal of pharmacology and experimental therapeutics,
N Speziale, and
E H Speziale, and
J M Pasquini
May 1998,
Biochemical and biophysical research communications,
N Speziale, and
E H Speziale, and
J M Pasquini
January 1967,
Physiologia Bohemoslovaca,
N Speziale, and
E H Speziale, and
J M Pasquini
February 1989,
Biochimica et biophysica acta,
N Speziale, and
E H Speziale, and
J M Pasquini
July 1978,
Prostaglandins,
N Speziale, and
E H Speziale, and
J M Pasquini
June 1986,
European journal of pharmacology,
N Speziale, and
E H Speziale, and
J M Pasquini
April 1981,
Archives of biochemistry and biophysics,
