Biomaterial Database

Formation of inositol phosphates and calcium mobilization in Swiss 3T3 cells in response to prostaglandin F2 alpha. 1985

T Sasaki

Signal transduction in the mitogenic action of prostaglandin F2 alpha on Swiss 3T3 cells has been studied. Confluent and quiescent Swiss 3T3 cells prelabeled with myo-[2-3H]inositol were stimulated with PGF2 alpha for 15 min at 37 degrees C in the presence of 5 mM LiCl, and the amount of total [3H]inositol phosphates, a sum of inositol tris-, bis-, and mono-phosphates, accumulated in the cells was determined. Addition of PGF2 alpha to the cells at 0.2 to 10 microM induced a 1.7 to 2.4-fold increase in [3H]-inositol phosphates. The accumulation was dose-dependent. Since assay of the agonist-dependent accumulation of inositol phosphates in the presence of LiCl has been used as a sensitive method for identifying those receptors that are coupled to the hydrolysis of phosphatidylinositol 4,5-bisphosphate [PtdIns (4,5)P2], these results indicate that PGF2 alpha induces in Swiss 3T3 cells hydrolysis of inositol lipids by a phospholipase C. The receptor-stimulated hydrolysis of PtdIns(4,5)P2 is usually coupled with a rise in cytoplasmic free Ca2+ concentration ([Ca2+]i). The effect of PGF2 alpha on [Ca2+]i was studied in quin-2 loaded Swiss 3T3 cells. On addition of 0.1 microM and 1 microM PGF2 alpha, there was an immediate increase in quin-2 fluorescence by 16 to 19% indicating a 1.5 to 1.8-fold increase in [Ca2+]i. These results therefore indicate that PGF2 alpha at 0.1 to 1 microM induces in Swiss 3T3 cells the hydrolysis of inositol lipids and a rise in [Ca2+]i.

UI	MeSH Term	Description	Entries
D007295	Inositol Phosphates	Phosphoric acid esters of inositol. They include mono- and polyphosphoric acid esters, with the exception of inositol hexaphosphate which is PHYTIC ACID.	Inositol Phosphate,Phosphate, Inositol,Phosphates, Inositol
D010716	Phosphatidylinositols	Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to the hexahydroxy alcohol, myo-inositol. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid, myo-inositol, and 2 moles of fatty acids.	Inositide Phospholipid,Inositol Phosphoglyceride,Inositol Phosphoglycerides,Inositol Phospholipid,Phosphoinositide,Phosphoinositides,PtdIns,Inositide Phospholipids,Inositol Phospholipids,Phosphatidyl Inositol,Phosphatidylinositol,Inositol, Phosphatidyl,Phosphoglyceride, Inositol,Phosphoglycerides, Inositol,Phospholipid, Inositide,Phospholipid, Inositol,Phospholipids, Inositide,Phospholipids, Inositol
D011453	Prostaglandins	A group of compounds derived from unsaturated 20-carbon fatty acids, primarily arachidonic acid, via the cyclooxygenase pathway. They are extremely potent mediators of a diverse group of physiological processes.	Prostaglandin,Prostanoid,Prostanoids
D011460	Prostaglandins F	(9 alpha,11 alpha,13E,15S)-9,11,15-Trihydroxyprost-13-en-1-oic acid (PGF(1 alpha)); (5Z,9 alpha,11,alpha,13E,15S)-9,11,15-trihydroxyprosta-5,13-dien-1-oic acid (PGF(2 alpha)); (5Z,9 alpha,11 alpha,13E,15S,17Z)-9,11,15-trihydroxyprosta-5,13,17-trien-1-oic acid (PGF(3 alpha)). A family of prostaglandins that includes three of the six naturally occurring prostaglandins. All naturally occurring PGF have an alpha configuration at the 9-carbon position. They stimulate uterine and bronchial smooth muscle and are often used as oxytocics.	PGF
D011464	Epoprostenol	A prostaglandin that is a powerful vasodilator and inhibits platelet aggregation. It is biosynthesized enzymatically from PROSTAGLANDIN ENDOPEROXIDES in human vascular tissue. The sodium salt has been also used to treat primary pulmonary hypertension (HYPERTENSION, PULMONARY).	Prostacyclin,Prostaglandin I2,Epoprostanol,Epoprostenol Sodium,Epoprostenol Sodium Salt, (5Z,9alpha,11alpha,13E,15S)-Isomer,Flolan,Prostaglandin I(2),Veletri
D011956	Receptors, Cell Surface	Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.	Cell Surface Receptor,Cell Surface Receptors,Hormone Receptors, Cell Surface,Receptors, Endogenous Substances,Cell Surface Hormone Receptors,Endogenous Substances Receptors,Receptor, Cell Surface,Surface Receptor, Cell
D002118	Calcium	A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.	Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D006868	Hydrolysis	The process of cleaving a chemical compound by the addition of a molecule of water.
D000634	Aminoquinolines	Quinolines substituted in any position by one or more amino groups.