Characterization of spermatogonial cells and niche in the scorpion mud turtle (Kinosternon scorpioides). 2019

G M J Costa, and A L Sousa, and A F A Figueiredo, and S M S N Lacerda, and L R França
Laboratory of Cellular Biology, Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.

Undifferentiated spermatogonia (Aund) or spermatogonial stem cells (SSCs) are committed to the establishment and maintenance of spermatogenesis and fertility throughout a male's life and are located in a highly specialized microenvironment called niche that regulates their fate. Although several studies have been developed on SSCs in mammalian testis, little is known about other vertebrate classes. The present study is the first to perform a more detailed investigation on the spermatogonial cells and their niche in a reptilian species. Thus, we characterized Aund/SSCs and evaluated the existence of SSCs niche in the Kinosternon scorpioides, a freshwater turtle found from Mexico to northern and central South America. Our results showed that, in this species, Aund/SSCs exhibited a nuclear morphological pattern similar to those described for other mammalian species already investigated. However, in comparison to other spermatogonial cell types, Aund/SSCs presented the largest nuclear volume in this turtle. Similar to some mammalian and fish species investigated, both GFRA1 and CSF1 receptors were expressed in Aund/SSCs in K. scorpioides. Also, as K. scorpioides Aund/SSCs were preferentially located near blood vessels, it can be suggested that this niche characteristic is a well conserved feature during evolution. Besides being valuable for comparative reproductive biology, our findings represent an important step towards the understanding of SSCs biology and the development of valuable systems/tools for SSCs culture and cryopreservation in turtles. Moreover, we expect that the above-mentioned results will be useful for reproductive biotechnologies as well as for governmental programs aiming at reptilian species conservation.

UI MeSH Term Description Entries
D008297 Male Males
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012605 Scorpions Arthropods of the order Scorpiones, of which 1500 to 2000 species have been described. The most common live in tropical or subtropical areas. They are nocturnal and feed principally on insects and other arthropods. They are large arachnids but do not attack man spontaneously. They have a venomous sting. Their medical significance varies considerably and is dependent on their habits and venom potency rather than on their size. At most, the sting is equivalent to that of a hornet but certain species possess a highly toxic venom potentially fatal to humans. (From Dorland, 27th ed; Smith, Insects and Other Arthropods of Medical Importance, 1973, p417; Barnes, Invertebrate Zoology, 5th ed, p503) Scorpion
D013020 South America The southern continent of the Western Hemisphere, extending southward from the Colombia-Panama border.
D013093 Spermatogonia Euploid male germ cells of an early stage of SPERMATOGENESIS, derived from prespermatogonia. With the onset of puberty, spermatogonia at the basement membrane of the seminiferous tubule proliferate by mitotic then meiotic divisions and give rise to the haploid SPERMATOCYTES. Spermatophores,Spermatogonias,Spermatophore
D013234 Stem Cells Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells. Colony-Forming Units,Mother Cells,Progenitor Cells,Colony-Forming Unit,Cell, Mother,Cell, Progenitor,Cell, Stem,Cells, Mother,Cells, Progenitor,Cells, Stem,Colony Forming Unit,Colony Forming Units,Mother Cell,Progenitor Cell,Stem Cell
D014426 Turtles Any reptile including tortoises, fresh water, and marine species of the order Testudines with a body encased in a bony or cartilaginous shell consisting of a top (carapace) and a bottom (plastron) derived from the ribs. Sea Turtles,Terrapins,Tortoises,Sea Turtle,Terrapin,Tortoise,Turtle,Turtle, Sea,Turtles, Sea
D015415 Biomarkers Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, ENVIRONMENTAL EXPOSURE and its effects, disease diagnosis; METABOLIC PROCESSES; SUBSTANCE ABUSE; PREGNANCY; cell line development; EPIDEMIOLOGIC STUDIES; etc. Biochemical Markers,Biological Markers,Biomarker,Clinical Markers,Immunologic Markers,Laboratory Markers,Markers, Biochemical,Markers, Biological,Markers, Clinical,Markers, Immunologic,Markers, Laboratory,Markers, Serum,Markers, Surrogate,Markers, Viral,Serum Markers,Surrogate Markers,Viral Markers,Biochemical Marker,Biologic Marker,Biologic Markers,Clinical Marker,Immune Marker,Immune Markers,Immunologic Marker,Laboratory Marker,Marker, Biochemical,Marker, Biological,Marker, Clinical,Marker, Immunologic,Marker, Laboratory,Marker, Serum,Marker, Surrogate,Serum Marker,Surrogate End Point,Surrogate End Points,Surrogate Endpoint,Surrogate Endpoints,Surrogate Marker,Viral Marker,Biological Marker,End Point, Surrogate,End Points, Surrogate,Endpoint, Surrogate,Endpoints, Surrogate,Marker, Biologic,Marker, Immune,Marker, Viral,Markers, Biologic,Markers, Immune
D016187 Receptors, Granulocyte-Macrophage Colony-Stimulating Factor Receptors that bind and internalize the granulocyte-macrophage stimulating factor. Their MW is believed to be 84 kD. The most mature myelomonocytic cells, specifically human neutrophils, macrophages, and eosinophils, express the highest number of affinity receptors for this growth factor. Antigens, CD116,CD116 Antigens,GM-CSF Receptors,CD116 Antigen,GM-CSF Receptor,Receptor, Granulocyte-Macrophage Colony-Stimulating Factor,Receptors, GM-CSF,Antigen, CD116,GM CSF Receptor,GM CSF Receptors,Receptor, GM-CSF,Receptor, Granulocyte Macrophage Colony Stimulating Factor,Receptors, GM CSF,Receptors, Granulocyte Macrophage Colony Stimulating Factor
D048429 Cell Size The quantity of volume or surface area of CELLS. Cell Volume,Cell Sizes,Cell Volumes,Size, Cell,Sizes, Cell,Volume, Cell,Volumes, Cell

Related Publications

G M J Costa, and A L Sousa, and A F A Figueiredo, and S M S N Lacerda, and L R França
April 2024, Open veterinary journal,
G M J Costa, and A L Sousa, and A F A Figueiredo, and S M S N Lacerda, and L R França
May 2020, Anatomical record (Hoboken, N.J. : 2007),
G M J Costa, and A L Sousa, and A F A Figueiredo, and S M S N Lacerda, and L R França
August 2020, Veterinary medicine and science,
G M J Costa, and A L Sousa, and A F A Figueiredo, and S M S N Lacerda, and L R França
December 2023, International journal for parasitology. Parasites and wildlife,
G M J Costa, and A L Sousa, and A F A Figueiredo, and S M S N Lacerda, and L R França
March 2013, Tropical animal health and production,
G M J Costa, and A L Sousa, and A F A Figueiredo, and S M S N Lacerda, and L R França
August 1994, Revista brasileira de biologia,
G M J Costa, and A L Sousa, and A F A Figueiredo, and S M S N Lacerda, and L R França
August 1996, Pigment cell research,
G M J Costa, and A L Sousa, and A F A Figueiredo, and S M S N Lacerda, and L R França
January 1994, Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology,
G M J Costa, and A L Sousa, and A F A Figueiredo, and S M S N Lacerda, and L R França
October 2022, The Journal of heredity,
G M J Costa, and A L Sousa, and A F A Figueiredo, and S M S N Lacerda, and L R França
February 2016, The Journal of parasitology,
Copied contents to your clipboard!