Biomaterial Database

Purification and preliminary characterization of phosphoglycerate mutase from Schizosaccharomyces pombe. 1985

N C Price, and D Duncan, and D J Ogg

Phosphoglycerate mutase could be purified to over 95% homogeneity by a single step procedure involving elution from Cibacron Blue-Sepharose by a pulse of cofactor 2,3-bisphosphoglycerate. Although the enzyme has been isolated in only small quantities (c. 100 micrograms), gel filtration and sodium dodecylsulphate polyacrylamide gel electrophoresis indicated that it is monomeric with Mr approximately 23,000, an extremely low value for this enzyme. Preliminary investigations of the kinetic characteristics and the nature of important amino acid side chains have been undertaken.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008970	Molecular Weight	The sum of the weight of all the atoms in a molecule.	Molecular Weights,Weight, Molecular,Weights, Molecular
D010736	Phosphoglycerate Mutase	An enzyme that catalyzes the conversion of 2-phospho-D-glycerate to 3-phospho-D-glycerate.	Glycerate (3-2)-Phosphomutase,Phosphoglyceromutase,Phosphoglycerate Phosphomutase,Mutase, Phosphoglycerate,Phosphomutase, Phosphoglycerate
D010770	Phosphotransferases	A rather large group of enzymes comprising not only those transferring phosphate but also diphosphate, nucleotidyl residues, and others. These have also been subdivided according to the acceptor group. (From Enzyme Nomenclature, 1992) EC 2.7.	Kinases,Phosphotransferase,Phosphotransferases, ATP,Transphosphorylase,Transphosphorylases,Kinase,ATP Phosphotransferases
D003931	Diacetyl	Carrier of aroma of butter, vinegar, coffee, and other foods.	2,3-Butanedione,Biacetyl,Diketobutane,Dimethyldiketone,Dimethylglyoxal,2,3 Butanedione
D004047	Diethyl Pyrocarbonate	Preservative for wines, soft drinks, and fruit juices and a gentle esterifying agent.	Diethyl Dicarbonate,Diethyl Oxydiformate,Pyrocarbonic Acid Diethyl Ester,Diethylpyrocarbonate,Ethoxyformic Anhydride,Anhydride, Ethoxyformic,Dicarbonate, Diethyl,Oxydiformate, Diethyl,Pyrocarbonate, Diethyl
D004718	Saccharomycetales	An order of fungi in the phylum Ascomycota that multiply by budding. They include the telomorphic ascomycetous yeasts which are found in a very wide range of habitats.	Budding Yeast,Endomycetales,Endomycopsis,Yeast, Budding,Budding Yeasts,Endomycetale,Endomycopses,Saccharomycetale,Yeasts, Budding
D012568	Schizosaccharomyces	A genus of ascomycetous fungi of the family Schizosaccharomycetaceae, order Schizosaccharomycetales.	Fission Yeast,Schizosaccharomyces malidevorans,Schizosaccharomyces pombe,Yeast, Fission,S pombe,Fission Yeasts
D013776	Tetrathionic Acid	A sulfuric acid dimer, formed by disulfide linkage. This compound has been used to prolong coagulation time and as an antidote in cyanide poisoning.	Sodium Tetrathionate,Potassium Tetrathionate,Tetrathionic Acid, Calcium Salt (1:1),Tetrathionic Acid, Dipotassium Salt,Tetrathionic Acid, Disodium Salt,Tetrathionic Acid, Disodium Salt, Dihydrate,Tetrathionate, Potassium,Tetrathionate, Sodium
D014639	Vanadium	A metallic element with the atomic symbol V, atomic number 23, and atomic weight 50.94. It is used in the manufacture of vanadium steel. Prolonged exposure can lead to chronic intoxication caused by absorption usually via the lungs.	Vanadium-51,Vanadium 51