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Murine LSTRA lymphoma cells contain a very active tyrosine protein kinase of 56 kDa (p56) which is not related to any of the other known tyrosine kinases. In the past the purification and characterization of the p56 have been hampered because of the low amount of this protein in LSTRA membranes. In this study, we have utilized a different approach for purification which consisted of trapping the protein in the membrane of vesicular stomatitis virus. Incubation of the virions with [gamma-32P]ATP resulted in the phosphorylation of p56 on tyrosine residues. Moreover, the phosphopeptide digest profile of vesicular stomatitis virus-p56 was identical to that observed with authentic LSTRA-p56. The p56 from such virions could be resolved from other proteins by two-dimensional gels, and furthermore, such virions have been used to prepare several antisera directed against the p56.
S Fischer, and
A Flamand, and
R Fagard, and
J F Chich, and
J P Piau, and
L Reibel, and
G Gacon
October 1970,
Virology,
S Fischer, and
A Flamand, and
R Fagard, and
J F Chich, and
J P Piau, and
L Reibel, and
G Gacon
March 1987,
Biochemical and biophysical research communications,
S Fischer, and
A Flamand, and
R Fagard, and
J F Chich, and
J P Piau, and
L Reibel, and
G Gacon
May 1973,
Comptes rendus hebdomadaires des seances de l'Academie des sciences. Serie D: Sciences naturelles,
S Fischer, and
A Flamand, and
R Fagard, and
J F Chich, and
J P Piau, and
L Reibel, and
G Gacon
August 1978,
Journal of virology,
S Fischer, and
A Flamand, and
R Fagard, and
J F Chich, and
J P Piau, and
L Reibel, and
G Gacon
November 1988,
Journal of virology,
S Fischer, and
A Flamand, and
R Fagard, and
J F Chich, and
J P Piau, and
L Reibel, and
G Gacon
January 1974,
Journal of virology,
S Fischer, and
A Flamand, and
R Fagard, and
J F Chich, and
J P Piau, and
L Reibel, and
G Gacon
August 1973,
Journal of virology,
S Fischer, and
A Flamand, and
R Fagard, and
J F Chich, and
J P Piau, and
L Reibel, and
G Gacon
January 1980,
Archives of virology,
S Fischer, and
A Flamand, and
R Fagard, and
J F Chich, and
J P Piau, and
L Reibel, and
G Gacon
February 1988,
European journal of biochemistry,
S Fischer, and
A Flamand, and
R Fagard, and
J F Chich, and
J P Piau, and
L Reibel, and
G Gacon
December 1984,
Molecular and cellular biology,
