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Human platelets washed and fixed in paraformaldehyde aggregate in the presence of the antibiotic ristocetin and normal plasma. This aggregation response is abolished after digestion of the fixed platelets with chymotrypsin. Antisera to fixed washed platelets were produced in rabbits and absorbed with chymotrypsin-treated, fixed washed platelets. Monovalent Fab fragments obtained from the isolated gamma-globulin fractions of the antisera blocked ristocetin-induced aggregation of fixed washed platelets in buffer and normal platelets in platelet-rich plasma. By double-antibody immunoprecipitation, it was shown that the antibody which blocked the ristocetin reaction interacted with a platelet membrane surface protein of mol wt 155,000. The results suggest that the glycoprotein I complex on the surface of the human platelet mediates ristocetin-induced von Willebrand factor-dependent platelet aggregation.
R L Nachman, and
E A Jaffe, and
B B Weksler
June 1979,
Nihon rinsho. Japanese journal of clinical medicine,
R L Nachman, and
E A Jaffe, and
B B Weksler
October 1975,
Blood,
R L Nachman, and
E A Jaffe, and
B B Weksler
April 1976,
Thrombosis and haemostasis,
R L Nachman, and
E A Jaffe, and
B B Weksler
February 1975,
American journal of clinical pathology,
R L Nachman, and
E A Jaffe, and
B B Weksler
November 1981,
British journal of haematology,
R L Nachman, and
E A Jaffe, and
B B Weksler
February 1989,
British journal of haematology,
R L Nachman, and
E A Jaffe, and
B B Weksler
September 1975,
Thrombosis et diathesis haemorrhagica,
R L Nachman, and
E A Jaffe, and
B B Weksler
June 1975,
Thrombosis research,
R L Nachman, and
E A Jaffe, and
B B Weksler
January 1977,
[Rinsho ketsueki] The Japanese journal of clinical hematology,
R L Nachman, and
E A Jaffe, and
B B Weksler
June 1975,
British medical journal,
