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In a search for exogenous promoters which function in cultured Drosophila cells, we have co-transfected a D. melanogaster cell line with an Epstein-Barr virus (EBV) cosmid clone which encodes the Epstein-Barr nuclear antigen (EBNA-1). Here we report that Drosophila cells containing stably integrated copies of EBNA-1 encoding DNA synthesise a polypeptide of mol. wt. identical to that of authentic EBNA-1, which is detectable with EBNA-positive but not EBNA-negative human serum. As in EBV-transformed lymphoblastoid cells, this neo-antigen is associated with the nucleus of transfected cells suggesting that cellular localisation signals which operate in mammalian cells are also recognised in insect cells.
M J Allday, and
J H Sinclair, and
A J MacGillivray, and
J H Sang
January 1982,
Progress in medical virology. Fortschritte der medizinischen Virusforschung. Progres en virologie medicale,
M J Allday, and
J H Sinclair, and
A J MacGillivray, and
J H Sang
December 1984,
Proceedings of the National Academy of Sciences of the United States of America,
M J Allday, and
J H Sinclair, and
A J MacGillivray, and
J H Sang
September 1987,
The Journal of general virology,
M J Allday, and
J H Sinclair, and
A J MacGillivray, and
J H Sang
January 1984,
The Annals of otology, rhinology, and laryngology,
M J Allday, and
J H Sinclair, and
A J MacGillivray, and
J H Sang
June 1983,
Science (New York, N.Y.),
M J Allday, and
J H Sinclair, and
A J MacGillivray, and
J H Sang
August 2000,
EMBO reports,
M J Allday, and
J H Sinclair, and
A J MacGillivray, and
J H Sang
April 1974,
Uirusu,
M J Allday, and
J H Sinclair, and
A J MacGillivray, and
J H Sang
January 1992,
Microbiology and immunology,
M J Allday, and
J H Sinclair, and
A J MacGillivray, and
J H Sang
February 2005,
Blood,
M J Allday, and
J H Sinclair, and
A J MacGillivray, and
J H Sang
January 1978,
Methods in cell biology,
