Biomaterial Database

Cloning and expression of an APH(3')-III phosphotransferase from Staphylococcus aureus in Streptomyces lividans. 1985

R Crameri, and J E Davies

An aminoglycoside 3' type III phosphotransferase derived from Staphylococcus aureus plasmid pRN1956 was cloned on the high copy number Streptomycetes vectors pIJ702 and pIJ704. Streptomyces lividans transformants carrying the hybrid plasmids show a resistance pattern towards aminoglycoside antibiotics comparable to the resistance pattern of S. aureus. The APH(3')-III with expanded spectrum of resistance, is a useful additional marker for gene cloning in Streptomycetes.

UI	MeSH Term	Description	Entries
D010770	Phosphotransferases	A rather large group of enzymes comprising not only those transferring phosphate but also diphosphate, nucleotidyl residues, and others. These have also been subdivided according to the acceptor group. (From Enzyme Nomenclature, 1992) EC 2.7.	Kinases,Phosphotransferase,Phosphotransferases, ATP,Transphosphorylase,Transphosphorylases,Kinase,ATP Phosphotransferases
D010957	Plasmids	Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.	Episomes,Episome,Plasmid
D003001	Cloning, Molecular	The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.	Molecular Cloning
D004352	Drug Resistance, Microbial	The ability of microorganisms, especially bacteria, to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).	Antibiotic Resistance,Antibiotic Resistance, Microbial,Antimicrobial Resistance, Drug,Antimicrobial Drug Resistance,Antimicrobial Drug Resistances,Antimicrobial Resistances, Drug,Drug Antimicrobial Resistance,Drug Antimicrobial Resistances,Drug Resistances, Microbial,Resistance, Antibiotic,Resistance, Drug Antimicrobial,Resistances, Drug Antimicrobial
D013211	Staphylococcus aureus	Potentially pathogenic bacteria found in nasal membranes, skin, hair follicles, and perineum of warm-blooded animals. They may cause a wide range of infections and intoxications.
D013302	Streptomyces	A genus of bacteria that form a nonfragmented aerial mycelium. Many species have been identified with some being pathogenic. This genus is responsible for producing a majority of the ANTI-BACTERIAL AGENTS of practical value.
D014169	Transformation, Bacterial	The heritable modification of the properties of a competent bacterium by naked DNA from another source. The uptake of naked DNA is a naturally occuring phenomenon in some bacteria. It is often used as a GENE TRANSFER TECHNIQUE.	Bacterial Transformation
D019868	Kanamycin Kinase	A class of enzymes that inactivate aminocyclitol-aminoglycoside antibiotics (AMINOGLYCOSIDES) by regiospecific PHOSPHORYLATION of the 3' and/or 5' hydroxyl.	Aminoglycoside Phosphotransferase,Neomycin Phosphotransferase,APH(3')-IIIa,APH(3')-IIb,APHVII,APT-3'-I and II,Amikacin 3'-Phosphotransferase,Aminocyclitol Phosphotransferase,Aminoglycoside 3'-Phosphotransferase Type VIII,Aminoglycoside 3'-Phosphotransferases (I and II),Aminoglycoside Phosphotransferase Type III,Kanamycin-Neomycin Phosphate Transferase,Neomycin Phosphotransferase II,aphVII Gene Product,3'-Phosphotransferase, Amikacin,APT 3' I and II,Amikacin 3' Phosphotransferase,Aminoglycoside 3' Phosphotransferase Type VIII,Kanamycin Neomycin Phosphate Transferase,Kinase, Kanamycin,Phosphate Transferase, Kanamycin-Neomycin,Phosphotransferase II, Neomycin,Phosphotransferase, Aminocyclitol,Phosphotransferase, Aminoglycoside,Phosphotransferase, Neomycin,Transferase, Kanamycin-Neomycin Phosphate