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[Isolation of viruses from water using porous silica]. 1985

I V Krasil'nikov, and V A Kazantseva, and O E Ivanova, and S S Savinskaia, and M S Balaian

The possibility of isolation of different viruses, hepatitis A virus among them, from water by means of adsorption chromatography on porous silica was demonstrated. Adsorption of viruses on the surface of silica depended on the kind of sorbent, water pH, and the rate of water flow through the column. The maximum elution of the adsorbed virus was observed using buffer solution with pH at least 11.0 and containing 1-2M NaCl. These conditions for adsorption-elution were found to be universal for different enterovirus types. In the field, the developed method was used for testing water specimens from various water bodies (sewage, river water, tap water). No viruses were found in 11 native specimens. Eluates of 4 out of 11 specimens were found to contain enteroviruses, hepatitis A virus among them.

UI MeSH Term Description Entries
D002845 Chromatography Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts. Chromatographies
D006863 Hydrogen-Ion Concentration The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000327 Adsorption The adhesion of gases, liquids, or dissolved solids onto a surface. It includes adsorptive phenomena of bacteria and viruses onto surfaces as well. ABSORPTION into the substance may follow but not necessarily. Adsorptions
D012822 Silicon Dioxide Transparent, tasteless crystals found in nature as agate, amethyst, chalcedony, cristobalite, flint, sand, QUARTZ, and tridymite. The compound is insoluble in water or acids except hydrofluoric acid. Silica,Aerosil,Aerosil 380,Cristobalite,Quso G-32,Quso G32,Tridymite,380, Aerosil,Dioxide, Silicon,G32, Quso,Quso G 32
D014780 Viruses Minute infectious agents whose genomes are composed of DNA or RNA, but not both. They are characterized by a lack of independent metabolism and the inability to replicate outside living host cells. Animal Viruses,Zoophaginae,Animal Virus,Virus,Virus, Animal,Viruses, Animal
D014871 Water Microbiology The presence of bacteria, viruses, and fungi in water. This term is not restricted to pathogenic organisms. Microbiology, Water

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