Biomaterial Database

Holistic Approaches in Lipid Production by Yarrowia lipolytica. 2018

Zbigniew Lazar, and Nian Liu, and Gregory Stephanopoulos

Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA; Department of Biotechnology and Food Microbiology, Wroclaw University of Environmental and Life Sciences, Chelmonskiego 37, 51-630 Wroclaw, Poland; These authors contributed equally. Electronic address: zbigniew.lazar@upwr.edu.pl.

Concerns about climate change have driven research on the production of lipid-derived biofuels as an alternative and renewable liquid fuel source. Using oleaginous yeasts for lipid synthesis creates the potential for cost-effective industrial-scale operations due to their ability to reach high lipid titer, yield, and productivity resulting from their unique metabolism. Yarrowia lipolytica is the model oleaginous yeast, with the best-studied lipid metabolism, the greatest number of genetic tools, and a fully sequenced genome. In this review we highlight multiomics studies that elucidate the mechanisms allowing this yeast to achieve lipid overaccumulation and then present several major metabolic engineering efforts that enhanced the production metrics in Y. lipolytica. Recent achievements that applied novel engineering strategies are emphasized.

UI	MeSH Term	Description	Entries
D001709	Biotechnology	Body of knowledge related to the use of organisms, cells or cell-derived constituents for the purpose of developing products which are technically, scientifically and clinically useful. Alteration of biologic function at the molecular level (i.e., GENETIC ENGINEERING) is a central focus; laboratory methods used include TRANSFECTION and CLONING technologies, sequence and structure analysis algorithms, computer databases, and gene and protein structure function analysis and prediction.	Biotechnologies
D050356	Lipid Metabolism	Physiological processes in biosynthesis (anabolism) and degradation (catabolism) of LIPIDS.	Metabolism, Lipid
D053898	Biosynthetic Pathways	Sets of enzymatic reactions occurring in organisms and that form biochemicals by making new covalent bonds.	Biosynthetic Pathway,Pathway, Biosynthetic,Pathways, Biosynthetic
D060847	Metabolic Engineering	Methods and techniques used to genetically modify cells' biosynthetic product output and develop conditions for growing the cells as BIOREACTORS.	Engineering, Metabolic
D025062	Yarrowia	A genus of ascomycetous yeast in the family Dipodascaceae, order SACCHAROMYCETALES.	Yarrowia lipolytica,Yarrowias