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We have investigated the effects of ovine corticotrophin releasing factor (oCRF) and its interaction with hydrocortisone (HC), and arginine vasopressin (AVP) on ACTH release from human corticotrophinoma cells in culture. Tumour tissue was obtained from six patients (three with active Cushing's disease and three with Nelson's syndrome). Cultures were maintained for periods of up to six months. Ovine CRF (21 nmol) significantly (P less than 0.01) stimulated ACTH release from all tumours. Dose response (21 pmol-21 nmol) effects were observed for the three tumours investigated over 2 and 4 h. Cortisol (20 mumol) significantly (P less than 0.01) inhibited basal ACTH release from one tumour (Nelson's syndrome) by 75% over 4 h, and completely prevented the stimulatory effects of oCRF. AVP directly stimulated ACTH release from two tumours (Nelson's syndrome), and also potentiated the stimulatory action of oCRF during 30 min incubations. These data show corticotrophinoma cells from subjects with Cushing's disease and Nelson's syndrome can be directly stimulated by hypothalamic oCRF and may be potentiated by AVP. Cortisol and oCRF have been shown in one tumour to have antagonistic actions at the pituitary level.
M C White, and
E F Adams, and
M Loizou, and
K Mashiter, and
R Fahlbusch
January 2012,
Endocrine journal,
M C White, and
E F Adams, and
M Loizou, and
K Mashiter, and
R Fahlbusch
May 1996,
The Journal of pharmacology and experimental therapeutics,
M C White, and
E F Adams, and
M Loizou, and
K Mashiter, and
R Fahlbusch
February 1994,
The Journal of endocrinology,
M C White, and
E F Adams, and
M Loizou, and
K Mashiter, and
R Fahlbusch
January 2004,
Drugs in R&D,
M C White, and
E F Adams, and
M Loizou, and
K Mashiter, and
R Fahlbusch
July 1984,
Neuroendocrinology,
M C White, and
E F Adams, and
M Loizou, and
K Mashiter, and
R Fahlbusch
October 1994,
The Journal of endocrinology,
M C White, and
E F Adams, and
M Loizou, and
K Mashiter, and
R Fahlbusch
August 1989,
Neuroendocrinology,
M C White, and
E F Adams, and
M Loizou, and
K Mashiter, and
R Fahlbusch
February 1984,
The Journal of clinical investigation,
M C White, and
E F Adams, and
M Loizou, and
K Mashiter, and
R Fahlbusch
June 1987,
Canadian journal of physiology and pharmacology,
M C White, and
E F Adams, and
M Loizou, and
K Mashiter, and
R Fahlbusch
August 2003,
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme,
