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The molecular forms of pro-opiomelanocortin in plasma of normal subjects and plasma and tissue extracts from patients with disorders of the hypothalamic-pituitary-adrenal axis have been characterized using gel filtration chromatography under acid dissociating conditions, and the molecular forms further investigated by high pressure liquid chromatography and affinity chromatography. A large molecular weight MSH/ACTH fragment was observed in all plasma samples chromatographed, although the proportions of this fragment were significantly greater in patients with the ectopic ACTH syndrome. A similar profile was observed in tumour extracts: a greater proportion of large molecular weight precursors was observed. Immunoreactive-gamma-MSH in extracts of ectopic tumours displayed marked and variable heterogeneity, and affinity chromatography with Concanavalin A-Sepharose indicated that this was due to differential glycosylation. High pressure liquid chromatography of a peak of amino terminal pro-opiomelanocortin (N-POC(1-76)) and 22 000 mol. wt MSH/ACTH indicated two peaks in each peptide, again possibly due to differential glycosylation. A possible neurointermediate lobe origin of atypical invasive pituitary tumours is discussed.
A C Hale, and
G M Besser, and
L H Rees
August 1988,
The Journal of endocrinology,
A C Hale, and
G M Besser, and
L H Rees
March 1983,
Clinical endocrinology,
A C Hale, and
G M Besser, and
L H Rees
March 1988,
The Biochemical journal,
A C Hale, and
G M Besser, and
L H Rees
March 1984,
Developmental biology,
A C Hale, and
G M Besser, and
L H Rees
January 1985,
Regulatory peptides. Supplement,
A C Hale, and
G M Besser, and
L H Rees
April 1983,
Regulatory peptides,
A C Hale, and
G M Besser, and
L H Rees
January 1986,
Neuroendocrinology,
A C Hale, and
G M Besser, and
L H Rees
January 1984,
Pharmacology & therapeutics,
A C Hale, and
G M Besser, and
L H Rees
March 2002,
Endocrinology and metabolism clinics of North America,
A C Hale, and
G M Besser, and
L H Rees
May 2010,
The Biochemical journal,
