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A novel, silica-based, reversed-phase column targeted to the separation of single-stranded oligodeoxyribonucleotides has been developed by grafting monochlorooctadecylsilane onto silica which is a base material of reversed-phase columns (TSK 120A and 120T), in which polychlorosilane is used as a grafting reagent. By applying a shallow gradient elution of an aqueous acetonitrile solution containing 0.1 M ammonium acetate, chromatography with this column produced well-resolved peaks for large samples such as those having bases up to 26.
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