To study the chemical constituents from Serissa japonica. The constituents were isolated by various chromatographic techniques and their structures were determined on the basis of spectroscopic data,as well as literatures. Eleven compounds were obtained and identified as 3-hydroxy-1,2-dimethoxyanthraquinone( 1),1,2,4-trimethoxy-3-hydroxy-6-methyl anthraquinone( 2),emodin( 3),pinoresinol( 4),medioresinol( 5),( 7S,8R)-balanophonin-4-O-β-D-glucopyranoside( 6),lupeol( 7),icariside F2( 8),gallic acid( 9),methyl caffeate( 10) and 4-hydroxy-3-methoxyphenyl-β-D-glucopyranoside( 11). Compounds 4and 5 are isolated from this plant for the first time,compounds 1 ~ 3 and 6 ~ 11 are isolated from Serissa genus for the first time.