-transparent.png){kind=logo}
An affinity adsorbent for ADP-ribosyltransferase (EC 2.4.2.30) has been synthesized by coupling 3-aminobenzamide to Sepharose 4B. Using this material, ADP-ribosyltransferase from human placenta has been purified from crude extract to homogeneity within a few hours. The enzyme has an apparent Km for NAD+ of 52 microM. Its molecular mass is 115,000 as determined by gel electrophoresis. The enzyme is DNA dependent and stimulated by histone, its temperature optimum is at 25 degrees C, and its pH optimum is around pH 9. alpha-NAD+, thymidine, caffeine, theophylline, theobromine, 3-methoxybenzamide, and nicotinamide inhibit the enzyme. Purification of ADP-ribosyltransferases from horse, rat, and chicken liver was also achieved with the method described.
H J Burtscher, and
B Auer, and
H Klocker, and
M Schweiger, and
M Hirsch-Kauffmann
January 1977,
Advances in experimental medicine and biology,
H J Burtscher, and
B Auer, and
H Klocker, and
M Schweiger, and
M Hirsch-Kauffmann
December 1983,
The Journal of biological chemistry,
H J Burtscher, and
B Auer, and
H Klocker, and
M Schweiger, and
M Hirsch-Kauffmann
July 1971,
Biochemistry,
H J Burtscher, and
B Auer, and
H Klocker, and
M Schweiger, and
M Hirsch-Kauffmann
January 1974,
Methods in enzymology,
H J Burtscher, and
B Auer, and
H Klocker, and
M Schweiger, and
M Hirsch-Kauffmann
November 1999,
The Journal of biological chemistry,
H J Burtscher, and
B Auer, and
H Klocker, and
M Schweiger, and
M Hirsch-Kauffmann
January 1974,
Methods in enzymology,
H J Burtscher, and
B Auer, and
H Klocker, and
M Schweiger, and
M Hirsch-Kauffmann
January 1974,
Biochimie,
H J Burtscher, and
B Auer, and
H Klocker, and
M Schweiger, and
M Hirsch-Kauffmann
June 1973,
Biochemical and biophysical research communications,
H J Burtscher, and
B Auer, and
H Klocker, and
M Schweiger, and
M Hirsch-Kauffmann
January 1975,
Biokhimiia (Moscow, Russia),
H J Burtscher, and
B Auer, and
H Klocker, and
M Schweiger, and
M Hirsch-Kauffmann
April 1978,
European journal of biochemistry,
