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The effect of cyclic GMP (cGMP) on human platelet activation was investigated, using its metabolically stable analogue, 8-bromo cGMP (8-bcGMP). Thrombin-induced serotonin secretion was inhibited by pretreatment with 8bcGMP in a dose-dependent manner. Production of inositol trisphosphate (IP3), a Ca2+ releaser was inhibited by 8bcGMP pretreatment of platelets. Preincubation of platelets with 8bcGMP was without effect on the basal level of cytosolic free Ca2+, measured by fluorescent indicator quin2, but suppressed its thrombin-induced enhancement independently of extracellular Ca2+. These results indicate that cGMP may be implicated in phospholipase C activation and Ca2+ mobilization (both influx through the plasma membrane and efflux from internal stores) in thrombin-activated human platelets.
S Nakashima, and
T Tohmatsu, and
H Hattori, and
Y Okano, and
Y Nozawa
January 1984,
Thrombosis research,
S Nakashima, and
T Tohmatsu, and
H Hattori, and
Y Okano, and
Y Nozawa
February 1990,
Biochemistry and cell biology = Biochimie et biologie cellulaire,
S Nakashima, and
T Tohmatsu, and
H Hattori, and
Y Okano, and
Y Nozawa
April 1983,
Biochemical and biophysical research communications,
S Nakashima, and
T Tohmatsu, and
H Hattori, and
Y Okano, and
Y Nozawa
June 1985,
Proceedings of the National Academy of Sciences of the United States of America,
S Nakashima, and
T Tohmatsu, and
H Hattori, and
Y Okano, and
Y Nozawa
October 1995,
Thrombosis research,
S Nakashima, and
T Tohmatsu, and
H Hattori, and
Y Okano, and
Y Nozawa
August 1996,
European journal of pharmacology,
S Nakashima, and
T Tohmatsu, and
H Hattori, and
Y Okano, and
Y Nozawa
June 1989,
Biochimica et biophysica acta,
S Nakashima, and
T Tohmatsu, and
H Hattori, and
Y Okano, and
Y Nozawa
August 1990,
Thrombosis and haemostasis,
S Nakashima, and
T Tohmatsu, and
H Hattori, and
Y Okano, and
Y Nozawa
October 1983,
Thrombosis research,
S Nakashima, and
T Tohmatsu, and
H Hattori, and
Y Okano, and
Y Nozawa
January 2007,
Thrombosis research,
