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The authors have examined the enzyme histochemical staining of surgically removed human thyroid tissue in an attempt to identify markers that might be useful in the histopathologic diagnosis of thyroid neoplasms. Fresh thyroid glands and other tissues were fixed in cold (4 degrees C) 4% paraformaldehyde and embedded in glycol methacrylate. Forty-two specimens were studied in thin sections, which gave excellent histologic detail and enzyme preservation. Cytologic detail was similar to that in Papanicolaou-stained smears, with good definition of nuclear inclusions and grooves, particularly in cases of papillary carcinoma. The enzyme histochemical reactions studied were as follows: adenosine triphosphatase, alkaline and acid phosphatases, alpha-naphthyl acetate esterase, and 5'-nucleotidase. Thyroid epithelial cells and the benign neoplasms derived from them were typically positive for 5'-nucleotidase, alpha-naphthyl acetate esterase, and acid phosphatase, and negative for adenosine triphosphatase and alkaline phosphatase. Staining for adenosine triphosphatase was present in papillary and follicular carcinomas and was seen in benign glands only under certain circumstances such as Graves' disease. The adenosine triphosphatase reaction therefore appears to be helpful in distinguishing between benign and malignant neoplasms derived from thyroid epithelium in humans and may be a useful adjunct to routine morphology.
M B Cohen, and
T R Miller, and
J H Beckstead
June 1963,
Archives of pathology,
M B Cohen, and
T R Miller, and
J H Beckstead
January 1975,
Acta histochemica. Supplementband,
M B Cohen, and
T R Miller, and
J H Beckstead
January 1975,
Acta histochemica. Supplementband,
M B Cohen, and
T R Miller, and
J H Beckstead
January 1982,
Zentralblatt fur allgemeine Pathologie u. pathologische Anatomie,
M B Cohen, and
T R Miller, and
J H Beckstead
July 1961,
The Journal of experimental zoology,
M B Cohen, and
T R Miller, and
J H Beckstead
September 1974,
Nihon Hinyokika Gakkai zasshi. The japanese journal of urology,
M B Cohen, and
T R Miller, and
J H Beckstead
February 1967,
Ugeskrift for laeger,
M B Cohen, and
T R Miller, and
J H Beckstead
January 1965,
Progress in brain research,
M B Cohen, and
T R Miller, and
J H Beckstead
January 1994,
Current therapy in endocrinology and metabolism,
