Controlling the lineshape of Fano resonance has great potential applications. Here we propose a type of acoustic Fano resonator, which is composed of a multi-layer zigzag line defects (ZLDs) sandwiched by double-layer zigzag steel rods in two-dimensional sonic crystals (SCs). We have theoretically and experimentally observed the asymmetric Fano resonances caused by the interference between the resonant and propagating self-collimated acoustic waves. It is demonstrated that the resonance dip frequency and Fano profile can be modulated by adjusting the structure parameters of the SC-based resonator. Our finding provides an efficient approach to manipulate sound propagation for future acoustic devices.