Biomaterial Database

The use of the single radial haemolysis technique in the serological diagnosis of dengue and Japanese encephalitis virus infections. 1985

Y C Chan, and H C Tan, and S H Tan, and K Balachandran

The single radial haemolysis test for the serological diagnosis of suspected dengue and Japanese encephalitis virus infections uses crude virus antigens with a haemagglutinin titre of 1:320 or 1:640. The results, which may be read 3 hours after the addition of a patient's serum, showed a general agreement between this test and haemagglutination-inhibition tests in the number of case diagnoses that were confirmed. The antibody responses of individual patients shown by the two tests, however, were different, which suggests that the two tests may not be measuring the same antibody. The single radial haemolysis test can distinguish between dengue and Japanese encephalitis viruses using specific mouse hyperimmune sera. Tests on a limited number of sera from Japanese encephalitis patients also showed no cross-reactions with dengue virus antigens in those cases having a low-titred but significant fourfold antibody rise to Japanese encephalitis antigen.

UI	MeSH Term	Description	Entries
D003715	Dengue	An acute febrile disease transmitted by the bite of AEDES mosquitoes infected with DENGUE VIRUS. It is self-limiting and characterized by fever, myalgia, headache, and rash. SEVERE DENGUE is a more virulent form of dengue.	Dengue Fever,Break-Bone Fever,Breakbone Fever,Classical Dengue,Classical Dengue Fever,Break Bone Fever,Classical Dengue Fevers,Classical Dengues,Dengue Fever, Classical,Dengue, Classical,Fever, Break-Bone,Fever, Breakbone,Fever, Dengue
D004672	Encephalitis, Japanese	A mosquito-borne encephalitis caused by the Japanese B encephalitis virus (ENCEPHALITIS VIRUS, JAPANESE) occurring throughout Eastern Asia and Australia. The majority of infections occur in children and are subclinical or have features limited to transient fever and gastrointestinal symptoms. Inflammation of the brain, spinal cord, and meninges may occur and lead to transient or permanent neurologic deficits (including a POLIOMYELITIS-like presentation); SEIZURES; COMA; and death. (From Adams et al., Principles of Neurology, 6th ed, p751; Lancet 1998 Apr 11;351(9109):1094-7)	Encephalitis, Japanese B,Japanese Encephalitis,Japanese B Encephalitis,Japanese B Viral Encephalitis,Viral Encephalitis, Japanese B
D006461	Hemolysis	The destruction of ERYTHROCYTES by many different causal agents such as antibodies, bacteria, chemicals, temperature, and changes in tonicity.	Haemolysis,Extravascular Hemolysis,Intravascular Hemolysis,Extravascular Hemolyses,Haemolyses,Hemolyses, Extravascular,Hemolyses, Intravascular,Hemolysis, Extravascular,Hemolysis, Intravascular,Intravascular Hemolyses
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012703	Serotyping	Process of determining and distinguishing species of bacteria or viruses based on antigens they share.	Serotypings